Perfusates collected from the caudal vena cava were collected after uterine perfusion and prepared for HPLC and LC/TOF-MS analysis.
METHODS: After performing uterine perfusion with BPA-GA in pregnant rats, we examined the expression and localization of the placental transporters for drug metabolites in the perfusate by reverse-transcriptase polymerase chain reaction and immunohistochemistry.
After being preserved in Ringer's lactate (RL) at 4 [degrees]C for 6 h ex vivo, the magnolol-treated grafts demonstrated significantly higher AST, ALT, and LDH levels in perfusates than those from negative controls.
The washout perfusates from livers were used for analysis of different liver injury-related biochemical parameters, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) that were determined using an automatic biochemical analyzer in Kaohsiung Chang-Gung Memorial Hospital.
In this regard, the ability of the former to secrete IGFBP-3 appears to be much greater than that of the latter to secrete other IGFBPs, because in our study, liver perfusates
of adult rats, like sera, contain greatest amounts of IGFBP-3 of all the secreted IGFBPs (Lee et al.
from the intervillous space were collected and stored at -70[degrees]C.
Both perfusates were maintained at a temperature of 37[degrees]C by a water bath (Glas water bath; UNI-GLAS, Zurich, Switzerland).
Furthermore, a venous drain, connected with a peristaltic pump (Reglo Digital; Ismatec, Glattbrug, Switzerland) removed the perfusate from the chamber and returned it to the maternal reservoir.
Crocin solution (50 [micro]g/ml) was prepared with different blank recirculated perfusates
and placed into a water bath at 37 [degrees]C for 2 or 4 h.
Original work by the Maastricht NHBD group has established threshold limits for tGST activity in kidney perfusates
for the selection of "viable" kidneys for transplantation (6).
through the probe membrane were collected at 20-mm intervals from 4 h after the insertion of the probe.
An improved and rapid HPLC-EC method for the isocratic separation of amino acid neurotransmitters from brain tissue and microdialysis perfusates