The washout perfusates from livers were used for analysis of different liver injury-related biochemical parameters, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) that were determined using an automatic biochemical analyzer in Kaohsiung Chang-Gung Memorial Hospital.
Since hepatocellular enzyme activities in washout perfusates after liver preservation reflect early graft viability (Smrekova et al.
Table 1 Biochemical analysis for the enzymatic activities in perfusates from liver grafts after cold preservation for 6h in RL solution with or without 10[micro]g/ml magnolol.
During in situ recirculation, the decreased amounts of crocin were similar to those of crocin when incubated with different blank recirculated perfusates in vitro (Tables 1 and 2).
The percent of drug remaining and drug lost in blank recirculating perfusates derived from different intestinal segments (mean[+ or -]SD, n = 6) Blank recirculated Drug remaining (%) perfusate 2h 4h Duodenum 93.
The concentrations of crocin in the perfusate were reduced through different intestinal segments, and the quantities of drug lost were greater throughout the colon.
Decisions for implantation were based on a combination of procurement and preservation characteristics, including tGST concentrations in kidney perfusates (10).
HFABP in the kidney perfusates was measured with a sandwich ELISA using a calibrator composed of recombinant human HFABP (12).
METHODS: After performing uterine perfusion with BPA-GA in pregnant rats, we examined the expression and localization of the placental transporters for drug metabolites in the perfusate by reverse-transcriptase polymerase chain reaction and immunohistochemistry.
In this perfusion system, the perfusate pumped into the abdominal aorta is circulated through one uterine artery-placenta-fetus-uterine vein unit and drips from the drain tube inserted in the caudal vein.
Both perfusates were maintained at a temperature of 37[degrees]C by a water bath (Glas water bath; UNI-GLAS, Zurich, Switzerland).
Furthermore, a venous drain, connected with a peristaltic pump (Reglo Digital; Ismatec, Glattbrug, Switzerland) removed the perfusate from the chamber and returned it to the maternal reservoir.