containing erythrocytes, such as blood/plasma (Patan et al., 2009) or blood/dialysate mixtures (Wagner et al., 2003), room air is adequate to achieve satisfactory saturation of the perfusate
while a low flow rate may support sufficient time for gas exchange in the oxygenation system (Wagner et al., 2003).
Each NMP technology works on similar principles but differs in terms of portability, degree of automation, substrate type and delivery, pressure and pulsatility of the recirculating perfusate
, and hepatic arterial and portal vein flow targets [37, 38].
Immediate and continuous perfusion minimizes the risk of microthrombi formation; however, thrombolytic medications could be supplemented to the perfusates
to dissolve any formed microthrombi.
This type of p[O.sub.2] electrode consists of an anode and cathode and measures oxygen tension amperometrically; the p[O.sub.2] electrode produces a current at a fixed polarizing voltage (-700 mV), which is directly proportional to the partial pressure of oxygen (p[O.sub.2]) in the perfusate
. The current evoked is a result of the subsequent reduction of oxygen at the cathode.
The rate of enzyme release into the perfusates
from isolated liver was calculated by means of the kinetics Enzfitter Program, based on the consideration of the simultaneity of enzyme release observed in isolated liver preparations .
When the microdialysis probe was placed in 200 pg/mL TNF-[alpha] solution and perfused at a flow rate of 1 [micro]L/min, the TNF-[alpha] concentration in the perfusates
gradually increased as a result of TNF-[alpha] crossing the membrane of microdialysis probe.
After being preserved in Ringer's lactate (RL) at 4 [degrees]C for 6 h ex vivo, the magnolol-treated grafts demonstrated significantly higher AST, ALT, and LDH levels in perfusates
than those from negative controls.
In this regard, the ability of the former to secrete IGFBP-3 appears to be much greater than that of the latter to secrete other IGFBPs, because in our study, liver perfusates
of adult rats, like sera, contain greatest amounts of IGFBP-3 of all the secreted IGFBPs (Lee et al., 1996).
from the intervillous space were collected and stored at -70[degrees]C.
Farias et al., "Comparing Normothermic Machine Perfusion Preservation with Different Perfusates
on Porcine Livers from Donors after Circulatory Death," American Journal of Transplantation, vol.
To ensure that we did not measure placental transfer of detached fluorescence dye, we wanted to recover the PS beads from the fetal (in case of normal perfusions) or the maternal (reverse perfusions) perfusates
after ex vivo perfusion.
During in situ recirculation, the decreased amounts of crocin were similar to those of crocin when incubated with different blank recirculated perfusates
in vitro (Tables 1 and 2).