Absolute recovery, expressed as the amount of di- and tripeptides sampled by induced diffusion across the microdialysis membranes per unit time (induced diffusive flux rates) measured relative to the standard solution, was strongly influenced by
perfusate flow rate.
Next to the hardware set-up, a multitude of different variables have to be defined as well, such as perfusion flow and pressure,
perfusate composition and oxygenation.
The
perfusate used in all studies was blood based using ABO-blood group O packed red blood cells; however, in the studies by Ravikumar et al., Bral et al., and Nasralla et al., the circuit and liver were additionally primed with gelatin-based plasma expander (Gelofusine[TM], B.
Bhangoo et al., "Associations of
perfusate biomarkers and pump parameters with delayed graft function and deceased donor kidney allograft function," American Journal of Transplantation, vol.
A single HF was adhered inside the shell and blocked at the
perfusate outlet with resin.
Following these baseline measurements, one group of pharmacologic agents was added to the
perfusate incontinuous infusion for 15-20 minutes and the experiment was repeated.
A pint of citrated blood was added to the
perfusate after 50 min.
A gas exchanger to remove carbon dioxide and provide oxygen to maintain these gases in the
perfusate at physiologic levels
P[O.sub.2], PC[O.sub.2], HC[O.sub.3], pH, and NO metabolites of the
perfusate were measured during steady-state and at the end of experiments (30 minutes).
Treatment of bladder tumors and benign prostatic hyperplasia with a new TUR system using physiological saline as
perfusate. World J Surg 2006; 30: 473-77.