was used in oxidizing the amino acids and was neutralized with sodium citrate dihydrate and then hydrolyzed with 6 N HCl for 22 hours at 110[degrees]C to be liberated from the protein.
Cysteine and methionine contents were determined by hydrolysis after oxidation with performic acid
High yield epoxidation of fatty acid methyl esters with performic acid
generated in situ.
A separate portion of the samples was oxidized with performic acid
and then hydrolyzed with 6 N HCl in order to obtain reliable cysteine and methionine values .
Probably, the most common derivatization involves conversion of the double bonds to epoxide groups using, for example, performic acid
The FAME was epoxidized at low temperature, with performic acid
generated in situ, using benzene as a diluent for the organic phase, to minimize epoxy ring opening (31).
Methionine and cystine were acid hydrolyzed by performic acid
Activity of performic acid
against spore-forming bacteria(a) Lot 1 Lot 2 Bacillus subtilis(b) 0/30 growth 0/30 growth B.
Methionine and cysteine were determined as methionine sulphone and cysteic acid using an AA Analyzer (Hitachi L-8900, Japan) after cold performic acid
oxidation overnight and hydrolyzing with 7.
During this step, the hydrogen peroxide reacts with the formic acid to form performic acid
Cysteine and methionine were analyzed after performic acid
oxidation and determined as cysteic acid and methionine sulfone, respectively.
Cystine was determined as cysteic acid and methionine as methionine sulphone after peroxidation with performic acid
and pre-column derivation using phenylisothiocyanate (L-8900, Automatic Amino Acid Analyzer; Hitachi, Japan).