feeding strategies, 37% increase in vulometric productivity was achieved (up to 0.861 /U/m/).
In vitro culture of tobacco callus on medium containing peptone
and phytate leads to growth improvement and higher genetic stability.
Afterwards, the solution was transferred to 90 ml of buffered peptone
water and incubated at 37degC for 18 hours.
SS agar: peptone
(5.0 g), meat extract (5.0 g), lactose (10.0 g), egg salts (8.5 g), natrium citrate (8.5 g), natrium thiosulphate (3.5 g), ferric citrate (1.0 g), agar (13.0 g), neutral red (0.023 g), brilliant green (0.00033 g).
Thus, constant rate of production of L-glutamic acid (6.8 mg/ml) was obtained experimentally up to sixteen subculture of Micrococcus glutamicus [AB.sub.100] studied before employed this strain into further optimization study using a maintenance medium composed of: glucose, 1.0%; peptone
, 0.5%; beef extract, 0.3%; yeast extract, 0.1% and agar 4.0% (as solidifying agent).
In accordance with the purpose of the test, diluted homogenous solutions of samples taken from those parts that are edible, were prepared with 0.1% buffered peptone
water: 25:225 for the Salmonella spp.
The isolation of this bacteria in medium considered to be selective by many authors did not occur because the Flavobacterium colonies were often prevented by the growth of other bacteria thus, modifications of the Anacker and Ordal medium (1955) as the replacement of tryptone by peptone
, beef extract by infusion of fish peptone
and the increase of sodium chlored, which were found to be very satisfactory for isolation of Flavobacterium columnare, because these inorganic substances provided optimal growth for Flavobacterium (Shieh, 1980).
A sample of the beef, (10.0g) was well minced in a 90 ml suitable diluent, SPS (Saline peptone
containing, g/L Distilled water; 1.0 g, Peptone
; 8.5 g, NaCl; pH 7.2 [+ or -]0.2 and autoclaved at 121 [degrees]C for 15 min.).
A 1 g subsample was transferred to 10 ml sterile 0.1% peptone
As Schwartz and his team infused glucose, a sugar, or peptone
, a protein, directly into the duodenum, they recorded both gut wall movements and the corresponding signals to the brain.
Samples from both sites contained strains capable of growth on crude oil as a sole source of carbon (unconditional strains) along with strains that could degrade oil if proteose peptone
was available in the growth medium (conditional strains).