However, rapidly growing mycobacteria can develop resistance by mutations in the peptidyltransferase
region of the 23S ribosome gene (24).
DON binds to RNA peptidyltransferase
site and inhibits protein synthesis (De Walle et al., 2010).
Ribosomal inactivation can be induced by a large family of ribonucleolytic proteins that cleave 28s ribosomal RNA at single phosphodiester bonds within a universally conserved sequence known as the sarcin-ricin loop, which leads to the dysfunction of peptidyltransferase
and subsequent global translational arrest [7,8].
T Caskey, "Peptidyltransferase
inhibition by trichodermin," The Journal of Biological Chemistry, vol.
Structure and function of rRNA in the decoding domain and at the peptidyltransferase
Kinetics of inhibition of rabbit reticulocyte peptidyltransferase
by anisomycin and sparsomycin.
The cycloheximide inhibits peptidyltransferase
, which is responsible for peptide bond synthesis during translation (26).
Erythromycin binds to the ribosome but, unlike larger macrolides, appears to cause dissociation of the peptidyl-tRNA, rather than blocking the peptidyltransferase