Peptide phage display | definition of peptide phage display by Medical dictionary
phage display (redirected from peptide phage display)
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A method of using genetically modified bacteriophages to make new proteins and replicate selected proteins, enabling the engineering of antibodies and the development of new drugs.
phage display A technique that fuses peptides to capsid proteins on the surface of a bacteriophage, which allows screening of libraries of phage-displayed peptides for binding to specific ligands. Identification of the DNA sequence of a selected phage identifies its peptide sequence.
phage display the display of recombinant PEPTIDES/PROTEINS on the surface of a PHAGE, for example M13, so that displayed proteins can be identified by their interaction with other proteins. The technique involves cloning the gene for the protein in a special phage cloning vector, such that the cloned gene is fused with the gene for a phage coat protein. The gene fusion directs synthesis of a hybrid coat protein, when the vector is propagated in an appropriate bacterial host. Upon assembly of the phages, this protein is inserted into the phage coat and hence displayed on the surface of phage particles. The procedure normally involves the use of PHAGE DISPLAY LIBRARY and rapid screening and selection of desired proteins. Uses include the engineering of a wide range of specific ANTIBODIES.
References in periodicals archive
Random peptide phage display
libraries have been successfully applied for mapping epitopes of various MAb specific to various pathogens, such as bovine herpes virus (Lehman et al.
Peptide phage display
technology is integral to this approach.