Inhibitory effects of pepstatin
A and mefloquine on the growth of Babesia parasites.
4), 1 mM EDTA, 1 mM EGTA, 5 [micro]g/ml aprotinin, 5 [micro]g/ml leupeptin, 5 [micro]g/ml pepstatin
A, 5 [micro]g/ml antipain, 50 mM NaF, 2 mM sodium orthovanadate, 10 mM sodium pyrophosphate, 0.
4) containing 1 mM EDTA, 1 mM dithiothreitol, 5 mg/L phenylmethylsulfonyl fluoride, 5 mg/L aprotinin, 5 mg/L chymostatin, and 5 mg/L pepstatin
added phosphatase inhibitor, sodium orthovanadate to a final concentration of 2 mM.
A, 1 mM phenylmethylsulforiyl fluoride, 1 [micro] g/mL aprotinin, 1 [micro] g/mL leupeptin, 1 [micro] g/mL pepstatin
, 1 mM [Na.
The cells were then treated with 1 ml of a 1% solution of Triton X-100 (containing 5 [micro]g/ml each of antipain, pepstatin
, and leupeptin as well as 1 mM phenylmethylsulfonyl fluoride) and kept at on ice for 20 min.
0004% w/v each of antipain, leupeptin, pepstatin
Samples to be analyzed for protease-sensitive PrP (PrPsen) contained the following protease inhibitors: 10 [micro]mol/L leupeptin, 1 [micro]mol/L pepstatin
A, and 1 [micro]g/mL aprotinin.
5 mmol/L p-nitrophenyl phosphate, 5 mmol/L glycerophosphate, 2 mmol/L EDTA, 2 mmol/L EGTA, 10 g/L aprotinin, 10 g/L pepstatin
, and 10 g/L leupeptin.
ECI's hypothesis is that ECI characterized Protector Proteins alphaA-Crystallin, gammaD-Crystallin and the chimeric protein, pepstatin
/leupeptin/alphaA-Crystallin, will inhibit the proteolytic enzymes secreted by the host and the pathogen, thus serving as a good therapeutic agent against the management of microbial keratitis.
2]) added protease inhibitors (2 mM [beta]-Mercaptoethanol, 3 [micro]/mL Leupeptin 1 [micro]g/mL Aprotinin 1 [micro]/mL Pepstatin
4], 10 mM [beta]-glycerophosphate, 5 [micro]g/m1 aprotinin, 1 [micro]g/ml leupeptin, 2 [micro]g/m1 pepstatin
A, and 1 mM phenylmethanesulfonyl fluoride (PMSF)], then were resuspended in buffer A containing 0.
Protease inhibitor cocktail III, nondetergent sulfobetaine-195 (NDSB-195), benzamidine, 1,10-phenan-throline, aprotinin, pepstatin
, and leupeptin were purchased from Calbiochem (La Jolla, CA).