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An inhibitor peptide from actinomycetes that inhibits pepsin and cathepsin D.
References in periodicals archive ?
1] in distilled water), N-tosyl-L-phenylalanine chloromethyl ketone (TPCK; 5mM in methanol), ethylenediamine tetra-acetic acid (EDTA; 10mM in distilled water) and pepstatin A (1mM in DMSO) were used for the analysis.
1 mM EDTA, 1 [micro]g/[micro]l leupeptin, 1 [micro]g/[micro]l pepstatin, 1 [micro]g/[micro]l aprotinin).
0) containing 5 [micro]g/mL of pepstatin A, 5 [micro]g/mL of aprotinin, and 100 mM of phenylmethylsulfonyl fluoride and were lysed by 3 freeze-thaw cycles.
75 pM aprotinin, 1 pM leupeptin, 1 [micro]M pepstatin, and 0.
Samples was homogenized in protein extraction buffer [2 mM EDTA, 100 uM phenylmethylsulfonyl fluoride (PMSF),10 ugmL-1 pepstatin A , 330 mM sorbitol, 10 ug/mL leupeptin and 50 mM HEPES/KOH pH 8.
1 mM phenylmethylsulfonyl fluoride, 5 [micro]g/mL aprotinin, 5 [micro]g/mL pepstatin A, and 1 [micro]g/mL chymostatin) and phosphatase inhibitors (5 mM [Na.
Inhibitory effects of pepstatin A and mefloquine on the growth of Babesia parasites.
1] pepstatin and quantified according to the Bradford dye-binding procedure (Bio-Rad, Hercules, CA, USA).
4) containing 1 mM EDTA, 1 mM dithiothreitol, 5 mg/L phenylmethylsulfonyl fluoride, 5 mg/L aprotinin, 5 mg/L chymostatin, and 5 mg/L pepstatin added phosphatase inhibitor, sodium orthovanadate to a final concentration of 2 mM.
3 g/1 g of tissue of 4-(2- aminoethyl) benzenesulfonyl fluoride, bestatin, pepstatin A, E-64, leupeptin, 1,10-phenanthroline (Sigma, St.