A, leupeptin, and aprotinin (all at 20 ng/ml, pH 7.
1] in distilled water), N-tosyl-L-phenylalanine chloromethyl ketone (TPCK; 5mM in methanol), ethylenediamine tetra-acetic acid (EDTA; 10mM in distilled water) and pepstatin
A (1mM in DMSO) were used for the analysis.
1 mM EDTA, 1 [micro]g/[micro]l leupeptin, 1 [micro]g/[micro]l pepstatin
, 1 [micro]g/[micro]l aprotinin).
0) containing 5 [micro]g/mL of pepstatin
A, 5 [micro]g/mL of aprotinin, and 100 mM of phenylmethylsulfonyl fluoride and were lysed by 3 freeze-thaw cycles.
75 pM aprotinin, 1 pM leupeptin, 1 [micro]M pepstatin
, and 0.
Samples was homogenized in protein extraction buffer [2 mM EDTA, 100 uM phenylmethylsulfonyl fluoride (PMSF),10 ugmL-1 pepstatin
A , 330 mM sorbitol, 10 ug/mL leupeptin and 50 mM HEPES/KOH pH 8.
1 mM phenylmethylsulfonyl fluoride, 5 [micro]g/mL aprotinin, 5 [micro]g/mL pepstatin
A, and 1 [micro]g/mL chymostatin) and phosphatase inhibitors (5 mM [Na.
A inhibited the total activities in stomach extracts in all juvenile stages (Fig.
Inhibitory effects of pepstatin
A and mefloquine on the growth of Babesia parasites.
and quantified according to the Bradford dye-binding procedure (Bio-Rad, Hercules, CA, USA).
4) containing 1 mM EDTA, 1 mM dithiothreitol, 5 mg/L phenylmethylsulfonyl fluoride, 5 mg/L aprotinin, 5 mg/L chymostatin, and 5 mg/L pepstatin
added phosphatase inhibitor, sodium orthovanadate to a final concentration of 2 mM.
3 g/1 g of tissue of 4-(2- aminoethyl) benzenesulfonyl fluoride, bestatin, pepstatin
A, E-64, leupeptin, 1,10-phenanthroline (Sigma, St.