Thus, using an in vivo model for the study of gastric secretion in rats with iv administration of several drugs, isoprenaline (isoproterenol), a [[beta].sub.1]- [[beta].sub.2]-adrenergic agonist, inhibited the acid secretion induced by pentagastrin
, an effect that was abolished by the administration of propranolol orbutoxamine (61).
After washing twice and adjusting the volume to 1.2 mL with DMEM containing 1% FBS, 150 [micro]L blocking solution (100-fold molar excess of pentagastrin
in PBS/0.5% BSA solution) was added to one-half while 150 [micro]L PBS/0.5% BSA was added to the other half of wells for volume compensation.
Cells were induced by DMEM/F12 basal medium (Gibco) supplemented with 10% FBS, 1% penicillin/streptomycin, 17.5 mM glucose, 25 ng/ml hEGF (Biological Industries, Kibbutz Beit Haemek, Israel), 10 mM nicotinamide (Sigma-Aldrich), 2nM activinA (Sigma-Aldrich), 10 nM exendin-4 (Sigma-Aldrich), 100 pM hHGF (Biological Industries), and 10 nM pentagastrin
(3) GLP-1 delays gastric emptying and inhibits pentagastrin
and acid secretion stimulated by food ingestion.
Lam examined the basal acid output (BAO) and the maximal acid output (MAO) after pentagastrin
stimulation, and fasting and postprandial gastrin levels in patients with LC.
Basal and pentagastrin
stimulated acid secretion in newborn human infants.
Alternatives to stimulation with pentagastrin
have been described (5, 10-12); considering its ubiquitous availability, stimulation with calcium (13, 14) is the most accessible and commonly used method.
In addition to these promising results their readings mirrored measurements obtainable through a pentagastrin
The false negativity of Meckel's scan may be attributed to an insufficient ectopic gastric mucosa, diluted radioactivity due to hemorrhage or intestinal hypersecretion, or inadequate patient preparation such as premedication with pentagastrin
, glucagon, and [H.sup.2] blocker to increase sensitivity of Meckel's scan .
Olbe, "Effect of omeprazole-a gastric proton pump inhibitor-on pentagastrin
stimulated acid secretion in man," Gut, vol.
Most of them found that all duodenal ulcer patients have raised maximal acid output (MAO), in response to histamine or pentagastrin
, showing a greater parietal cell mass (Blair and others 1987).