The main enzyme activities in a commercial pectinase are pectin lyase
, pectin methyl-esterase and polygalacturonase.
Selective and sensitive detection of pectin lyase
activity using a colorimetric test: application to the screening of microorganisms possessing pectin lyase
Alana et al., (1990)  reported that Ca2+, Mg2+, Zn2+, and Mn2+ did not affect pectin lyase
activity of P.
Pectinases classified on the basis of their mode of action to polygalacturonase (EC 126.96.36.199), pectin esterase (EC 188.8.131.52), pectin lyase
(EC 184.108.40.206) and pectate lyase (EC 220.127.116.11).
(a) Pectin lyase
(PL) activity was assayed spectrophotometrically by determining uronide at 235 nm .
Pectinases include polygalacturonase (EC 18.104.22.168), pectin esterase (EC 22.214.171.124), pectin lyase
(EC 126.96.36.199) and pectate lyase (EC 188.8.131.52), classified on the basis of their mode of action [6, 7 & 8].
Scouring process called "Bioscouring" was recently developed using alkaline pectin lyase
BioPrep isolated by Novozymes.
production by a Penicillium italicum strain.
Pectinases were broadly classified into polygalacturonase, pectin esterase and pectin lyase
. Polygalacturonase (PG) acts on the pectin chain by adding a water molecule to break the linkage between two galacturonan molecules; Pectin lyase
(PL) acts on the galacturonan chain by removing a water molecule from the linkage to break it and to release its products; whereas, Pectin esterase esterifies the pectin by removing methoxy esters (3).
Lyases that act on polygalacturonic acid known as pectate lyase, while those act on pectin called pectin lyase
, which are widely distributed in various families of microorganisms  and are produced by bacteria especially Bacillus sp.
Firstly, pectinase is not a single enzyme, but, rather, a set of several pectin-splitting substances: pectinesterases, polygalacturonases, pectin lyases
, and pectate lyases.