As a result, the use of early passage cells
is recommended; however, early passage cell
populations have increased likelihood of heterogeneity whilst late passage cells
retain characteristic markers for MSC phenotype in a selectively more homogenous population [36, 37].
Total RNA was extracted from different passage cells
by using RNeasy Mini Kits (Qiagen, Germany), and then cDNA was synthesized using Thermo Scientific RevertAid First Strand cDNA Synthesis Kit (Thermo, USA).
Area under the curve (AUC) was calculated using trapezoid formula in order to compare the effect of cell density (for early and late passage cells
) on collagen contraction.
This has been recommended in previous best practice documents to passage cells
for no more than fifteen passages or for a maximum of three monthsfby WHO guidance (WHO, 2013).
Cytometers easily integrate with high-quality liquid handling platforms and are critical in monitoring cell health and viability, and in determining cell confluence, which indicates when to passage cells
Some short cells without deposition of suberin lamellae are called passage cells
, and may be responsible for symplast control, and transference of water and nutrients (Peterson & Enstone 1996, Ma & Peterson 2003).
In this study the intense of glycosaminoglycan was more pronounced in the early versus late passage MSCs, indicating an enhanced chondrogenesis in the early passage cells
Adipogenic differentiation was induced in the third passage cells
by plating the EnSCs at 2 x [10.sup.4] cells per [cm.sup.2], allowing the cells to reach confluence and then incubating for a further 48 hr.
Available as untreated and growth arrested by Mitomycin C or irradiation, AMSBIO feeder cells are manufactured using a standardized production process to yield low passage cells
which consistently maintain ES and iPS cell cultures in the undifferentiated state.
At every third passage cells
on attaining confluency when stained with X-gal, it was observed that 0.01% of the cell population was senescent.
RNA samples were extracted and purified from 150-[micro]L lysates of primary cells and four passage cells
and used as templates for RT-PCR analysis to determine the presence of TSV.