partial digestion

partial digestion

limited (incomplete) digestion of a molecule. For example, in the case of partial digestion of DNA with a RESTRICTION ENZYME, conditions such as limited time of incubation or limited enzyme concentration are provided, that permit only a fraction of the total number of restriction sites in individual molecules to be cleaved.
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Fosmid cloning allows high-efficiency cloning of target genomic structures by partial digestion. It hasan advantages of being double cos site vector.
subtilis G8 keratinolytic enzyme, partial digestion by bacterial culture was simpler, lower production cost and no significant difference in total crude protein content.
pGEM-T Easy/CD19 gene construct was double digested using 10 units of each KpnI (as partial digestion for 5 min) and HindIII (as complete digestion for 16 hr) restriction enzymes (Thermo Fisher Scientific, Inc., MA, USA).
Mixing and partial digestion of the food in the stomach by its gastric secretions produce a pulpy fluid mix called chyme.
Even after partial digestion, Ctenophora in spiny dogfish stomachs were identifiable, particularly the ctene.
The work, Still and Chew, got Latham in hot water professionally but demonstrated that something interesting could be made via a process of partial digestion. Adamo's mounds are inspired by another, more prosaic (though in some ways equally sophisticated) use of gnawed wood but lack the types of allusive trains of thought and event that came together in Latham's subversive project--and feel distinctly raw by comparison.
However, because there is no methylase in the PCR reaction system, the partial digestion probably is due to hybridization between related species and these results are similar to past research on bacteria (Hong et al., 1999) and fungi (Thomas et al., 1996).
The partial digestion of fats by the mother's body means that the baby can absorb them using less valuable energy than is needed to digest formula.
This step is followed by ligation of a specific indexer, complementary to the cut site determined in a previous step, to one of the products of partial digestion. In each round of indexing, DNA processed in the previous cycle is used.
When you drink fluids with meals, you drown your digestive enzymes and only partial digestion takes
The size of prey consumed by pinnipeds can usually be reliably estimated from otoliths recovered in scats if partial digestion is accounted for (Tollit et al., 1997).
When we drink fluids with meals, we drown digestive enzymes and only partial digestion takes place.