was determined by counting the number of parasite against 200 erythrocytes.
The effects of the extract on parasitaemia
level in this study are similar to the ones reported by previous studies such as on Asparagus africanus (Dikasso et al.
The clinical correlations with malaria parasitaemia
grading values indicate that at the low levels observed patients may be asymptomatic, and this may explain why our participants had no clinical signs of malaria.
Malaria in areas of unstable transmission usually follows seasonal patterns of transmission as mosquito populations fluctuate, with the prevalence of parasitaemia
at a minimum in the cooler dry season.
In our case, the patient did not have any features to characterize severe malaria, and parasitaemia
and gametocyte carriage in Eastern Sudan.
The level of parasitaemia
was determined by Giemsa stained blood smear.
The parasite was then allowed to incubate in the mice for 72 hours after which thin blood film was prepared from tail snip, and stained using Giemsa stain and the parasitaemia
confirmed by viewing using x100 objective of the light microscope.
RDTs have high sensitivity and specificity (>90%) at a parasitaemia
>100 asexual parasites/[micro]l [39,4].
The described method is limited by the required prior knowledge of the erythrocyte area information which is needed for the parasitaemia
The percentage parasitaemia
was calculated as; % parasitaemia
= (parasite per microlitre of blood/4,000,000) x 100, where 4,000,000 red blood cells (RBC) were assumed per microliter of blood (7).