The polymerase product is thus visualized and quantified chain as peak areas of respective length using an reaction automated DNA sequencer with Gene Scan software Paralogous A PCR-based method for the detection of sequence targeted chromosome number abnormalities, quantification based on the use of paralogous genes
. Paralogous sequences have high degree of sequence identity but accumulate nucleotide substitution in a locus specific manner.
Kuipers, "Differential Expression of Two Paralogous Genes
of Bacillus subtilis Encoding Single-Stranded DNA Binding Protein," Journal of Bacteriology, vol.
Tandem gene duplicates were defined as paralogous genes
located within 50 kb and separated by fewer than five nonhomologous spacer genes .
Trinity partitions the sequence data into many individual de Bruijn graphs, each representing the transcriptional complexity at a given gene or locus, and then processes each graph independently to extract full-length splicing isoforms and to tease apart transcripts derived from paralogous genes
. The sequences produced by Trinity are called unigenes.
Thus, these studies show that the normal patterning of lumbar and sacral vertebrae as well as the changes in the axial pattern, such as LSTV, result from mutations in the Hox-10 and Hox-11 paralogous genes
Interactions between miRNAs and complementary sequences on genes and pseudogenes that share sequence homology are thought to be a back channel of communication regulating expression levels between paralogous genes
. Given the name "competing endogenous RNAs (ceRNAs), these miRNAs bind to miRNA response elements on genes and pseudogenes, providing another explanation for the persistence of non-coding DNA .
This new rule crucially substitutes the concept of ontogenetic transformation by the more general concept of homology and additive phylogenetic change, which can be applied to cases in which homologous entities accumulate "iteratively" in evolution (e.g., generation of paralogous genes
The classic concept of the functional consequences of gene duplication, proposed by Susumu Ohno, holds that duplication produces two functionally redundant, paralogous genes
and, thereby, frees one of them from selective constraints.
Due to an evolutionary conservation of the BAG domain, putative orthologous and/or paralogous genes
have been identified using homologous analysis in yeast, animals and plants (Takayama et al., 1999; Coulson et al., 2005; Doukhanina et al.
The normal patterning of lumbar and sacral vertebrae as well as the changes in the axial pattern, such as lumbosacral transitional vertebra, results from mutations in the HOX- 10 and HOX-11 paralogous genes
However, assembly and interpretation must be handled cautiously, as paralogous genes
may lead to misassemblies of sequences, particularly in polyploid species such as wheat .
Analyzing the paralogous genes
of cognitive impairment, some autosomal genes showed their paralogs on other autosomes and was involved in MR, e.g.