Into 250 mL round-bottom flask, bisphenol-a (4.57 g, 100.0 mmol), aniline (3.65 g, 100 mmol), paraformaldehyde
(2.5531 g, 400 mmol) were added.
After washing with phosphate buffer 0.1 M (pH 7.4) samples were fixed in 0.1% glutaraldehyde and 2% paraformaldehyde
in phosphate buffer 0.1 M (pH 7.4) for 1.30 hour at 4[degrees]C.
The thyroid tissue was immersion-fixed in 4% paraformaldehyde
at room temperature and then processed into paraffin sections by using a routine procedure.
Teratocytes encased between two Formvar layers were fixed in 2% glutaraldehyde/2% paraformaldehyde
in 0.1 M cacodylate buffer.
(99%), toluene (99.8%), and toluene diisocyanate (2,4 isomer = 80%; 2,6 isomer 20%) from Merck; and 1,4 dioxane (98%) from Riedel-de-Haan were used as received.
We collected the brains and postfixed them for 24 h in 4 percent paraformaldehyde
followed by cryoprotection in 30 percent sucrose in PBS overnight.
Then 4% paraformaldehyde
(4[degrees] C) was added into each well (300 [micro]L/well) for 15 minutes.
Monolayers of P3X63Ag.653 cell line were fixed according to the following fixation protocols: 4% neutral buffered formaldehyde (Spectrum, F0110) and 4% paraformaldehyde
(BDH, 294474L) for 30 and 20 minutes at room temperature, respectively, and acetone (Spectrum, A1020), ethanol (Spectrum, E1028), and methanol (Spectrum, M1240) for 10 minutes at 2-8[degrees]C each one.
The resin reacts with methylene donors such as formaldehyde, paraformaldehyde
, hexamethylene-tetramine, hexamethoxymethylmelamine and 2-nitro-2-methyl-l-propanol to become a thermoset.
Methods: E11.5 mouse embryos expressing all or only single VEGF isoforms were fixed in 3.7% buffered paraformaldehyde
, equilibrated in 30% sucrose and cryosectioned at 30 [micro]m.
Ovaries dissected from the queens were fixed in 4% paraformaldehyde
; in 0.1 M, pH 7.4 phosphate buffer during 24 hours.
A collection of planulae and juvenile polyps were fixed in ice-cold 3.7% paraformaldehyde
(Fisher)/0.2% gluteraldehyde (Sigma) in 1/3 ASW for 90 s, then postfixed in ice-cold 3.7% paraformaldehyde
in 1/3 ASW for 15 min prior to storage in 100% methanol (Fisher) at--20 [degrees]C.