After several rinses in PBS, specimens were fixed in fresh 4% paraformaldehyde
solution in PBS, pH 7.
5% glutaraldehyde and 2% paraformaldehyde
(1:1) in 0.
Approaches using added paraformaldehyde
According to the report, the global paraformaldehyde
market was valued at US$ 468.
Brains were sliced coronally into 2 mm-thick slices and stained with 1% TTC for 15 min at 37[degrees]C and then fixed in 4% paraformaldehyde
3]) monomer were synthesized based on classical benzoxazine system using on bisphenol A and paraformaldehyde
After treatment of SOE, PFFs were placed onto a cover slip by centrifuge (Centurion Scientific Ltd, UK), fixed 4% paraformaldehyde
solution for 15 min and then washed three times with PBS-T (0.
9,9-bis(4-hydroxyphenol)fluorene (BHPF) (98%), 4,4-diaminodiphenylmethane (MDA) (99%), paraformaldehyde
(96%), allyamine (99%), and bisphenol A (99%) were purchased from Aladdin, aniline, xylene (boiling point: 137-142[degrees]C) were received from Tianjin Chemical.
We fixed cells by incubation with 2% paraformaldehyde
in PBS for 20 min.
2]O), Ammonium dibasic phosphate, Polyethylene glycol, Calcium Chloride, Methanol, Minimum essential medium (MEM), Acetic acid, Paraformaldehyde
were purchased from SIGMA Aldrich Company, Glutaraldehyde and Hen Lysozyme were purchased from Fluka.
Thereafter, a fixative of 2 [micro]L of 1% paraformaldehyde
in phospate-buffered saline was added, followed by incubation at 5[degrees]C for 30 minutes.
After lectin labeling, samples were washed and fixed 1:1 with paraformaldehyde