2] of 1 mol of reduced glutathione to oxidized glutathione
per minute at 25[degrees]C, pH 7.
Elevated Glutathione: The study reported in the third column shows that people taking ME-3 had a remarkable 49% increase in the ratio of reduced to oxidized glutathione
One molecule of oxidized glutathione
is consumed for each molecule reduction of oxidized glutathione
During the action of glutathione reductase, glutathione is regenerated and converted to oxidized glutathione
that turns NADPH to glutathione again by glutathione reductase (2).
Regeneration of ascorbate was mediated by dehydroascorbate reductase (DHAR) and stimulated by oxidation of glutathione (GSH) to become oxidized glutathione
The amount of reduced glutathione (GSH) and oxidized glutathione
(GSSG) was measured with the GSSG/GSH Quantification Kit (Dojindo), following the manufacturer's instructions.
Levels of reduced (GSH) and oxidized glutathione
(GSSG), total glutathione, reduced and oxidized glutathione
ratio, and non-protein thiols in K.
16] Glutathione peroxidase catalyzes the decomposition of hydrogen peroxide in presence of reduced glutathione forming oxidized glutathione
thiobarbituric acid (TBA), reduced glutathione (GSH), oxidized glutathione
(GSSG) and dithio-bis-2nitrobenzoic acid [DTNB] were purchased from Sigma Chemicals Co.
Uncoupling can also occur as a result of various other factors, one of which is from high levels of oxidized glutathione
(GSSG) relative to reduced glutathione (GSH), as oxidized glutathione
lowers the body's defense against free radicals.
Glucose-6-phosphate dehydrogenase enzyme maintains a continuous supply of reduced nicotinamide adenine dinucleotide phosphate (NADPH) which in turn is essential for regeneration of reduced glutathione from oxidized glutathione
(Gahlawat et al.
Glutathione is principally important naturally occurring antioxidant in semen, having low molecular weight, can easily mobilize the system for the removal of peroxides by a reaction that results in the generation of oxidized glutathione
(Meister and Anderson, 1983).