thiobarbituric acid (TBA), reduced glutathione (GSH), oxidized glutathione
(GSSG) and dithio-bis-2nitrobenzoic acid [DTNB] were purchased from Sigma Chemicals Co.
2] and other peroxides that are formed on lipids within the body to yield water and oxidized glutathione
was calculated by subtracting the value of glutathione reduced from total glutathione.
The plaintiffs alleged that the defendants made materially false and misleading statements and omissions regarding the progress of the Phase 3 clinical trial before the United States Food and Drug Administration of Novelos' oxidized glutathione
compound, NOV-002, in application to non-small cell lung cancer.
Its protective action is based on oxidation of the thiol group of its cysteine with the formation of oxidized glutathione
(GSSG) (Tzeng et al.
9 percent lower than controls, with oxidized glutathione
levels 125 percent higher, indicating oxidative stress.
NADPH is required as a cofactor in the reduction of oxidized glutathione
(GSSH) to its reduced state (GSH) and for the reduction of mixed disulfides of glutathione and cellular proteins (6, 10).
28 mM) the oxidized glutathione
is immediately converted to the reduced form with a concomitant oxidation of NADPH to NADP+.
In erythrocytes, NADPH is necessary to convert oxidized glutathione
into its reduced form.
Samples for reduced and oxidized glutathione
(GSH, GSSG, respectively) were stored at -70[degrees]C until the analysis.
In this study we determined reduced glutathione, oxidized glutathione
(GSSG), glutathione peroxidase (GPx), glutathione reductase (GR), and glucose-6-phosphate dehydrogenase (G6PDH) in skeletal muscle and heart of young and aged rats.
Interestingly, we can also note a reduction in the level of oxidized glutathione
," reported Sophie Lafay, Scientific Director at Berkem.