chromosome walking

(redirected from overlap hybridization)
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chro·mo·some walk·ing

sequential isolation of overlapping sequences of DNA (that is, clones); with this procedure large regions of the chromosome can be spanned.

chromosome walking

a molecular genetic technique by which overlapping molecular clones that span large chromosomal intervals are isolated.

chromosome walking

A procedure for determining the nucleotide sequence of segments of DNA that are located on either side of a region of fully characterised (i.e., “sequenced”) DNA. Chromosome walking requires the dividing (“walking out”) of each segment of DNA into overlapping short (15–20,000) bases from a point of a known sequence; this enables one to sequence DNA far removed from a marker gene.

chro·mo·some walk·ing

(krō'mŏ-sōm wawk'ing)
A process of extending a genetic map by successive hybridization steps.

chromosome walking

a technique for mapping chromosomes that involves the sequential isolation of clones carrying overlapping NUCLEOTIDE SEQUENCES. This makes it possible to ‘walk’ along the chromosome from one gene to the next, to reach a desired LOCUS. Chromosome walking is used in genome sequencing projects, see CLONE CONTIG SEQUENCING METHOD.