The aglycon obtained from the hydrolysis of the cardiac glycoside, ouabain; exerts cardiotonic activity.
References in periodicals archive ?
Ouabain (G-strophanthin) octahydrate, ouabagenin, dihydroouabain, digoxin, digitoxin, digoxigenin, and other related steroids (aldosterone, bufalin, cortisone, hydrocortisone, progesterone, [beta]-estradiol, estrone, testosterone, and dehydroepiandrosterone), bovine thyroglobulin, and sodium cyanoborohydride were obtained from Sigma.
Ouabagenin showed the highest cross-reactivity (52%), followed by digoxin (1.
This is supported by the cross-reactivity profile of the ouabain antiserum (a-ouabain-199) raised against the ouabain-thyroglobulin conjugate, which demonstrated the highest cross-reactivity (52%) to the aglycone ouabagenin.
The rhamnose moiety, which is absent from ouabagenin, is less important, leading to only a partial reduction in immunoreactivity.
3 Digitoxin 78 Ouabagenin 55 Strophanthidin 47 Dihydroouabain 0.