Lee et al., "Osteogenic stimulation
of human adipose-derived stem cells by pre-treatment with fibroblast growth factor 2," Cell and Tissue Research, vol.
The investigated markers were detected in the supernatants collected at the last day of the differentiation process, the 18th day of osteogenic stimulation and the 12th day of adipogenic stimulation.
After osteogenic stimulation, on the 18th day of culture, the cultures were rinsed with HBSS and lysed in a culture dish with 0.5 mL of TRI Reagent[R].
Osteogenic stimulation of EPC led to a decrease in vWF gene expression (P = 0.09, Figure 6(a)).
They described the osteogenic stimulation of MSCs which were cocultured with endothelial cells and identified BMP2 released by the endothelial cells as a responsible factor for the osteogenic differentiation .
Additionally, the upregulation ([2.sup.-[DELTA][DELTA]Ct]) of the ALPL (alkaline phosphatase) gene in response to osteogenic stimulation was also significant in relation to PD-BS (r = 0.470, p = 0.020), but not PD (r = 0.026).
When considered at all time points, osteogenic stimulation caused the upregulation of several (24) genes, but only four were reduced (Table 1).