Analysis of recombinant human growth hormone directly in osmotic shock fluids.
The release of enzymes by osmotic shock from Escherichia coli in exponential phase.
The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts.
We investigated the effects of hemolysis on plasma ammonia measurement using the osmotic shock protocol versus the shearing method at 2 different ammonia concentrations on the Roche Integra 800.
To determine if hemolysate aging resulted in ammonia production, an EDTA whole blood sample was treated per osmotic shock protocol to prepare the hemolysate, except that the hemolysate was split into 2 portions after the addition of water and before freezing.
Periplasmic expression has several advantages over the cytosolic one as follows: i) it simplifies protein purification, ii) it maintains an oxidizing environment to promote proper protein folding, removal of signal peptide and elimination of polypeptides, iii) the outer membrane can be selectively degraded by simple osmotic shock, iv) the periplasm is accessible to molecules, e.
Periplasmic osmotic shock was carried out as described previously (19).
The periplasmic content was released from the bacterial pellet by osmotic shock.
By creating an osmotic shock
around the site of the injection, we create an inflammatory response in the tendon, which may be absent in some patients.
This elevation in mesogleal potassium concentration was seen only after the larger salinity change (20 [per thousands] to 8 [per thousands]), and it is possible that osmotic shock may have resulted in a transient increase in cellular potassium permeability or, perhaps, in some cell damage leading to potassium contamination of the mesogleal fluid.
It is also possible that tissue permeability to these ions changes after the initial osmotic shock.
The final salinity level was achieved by raising 100 mmol/L NaCl every 24 h in order to avoid osmotic shock