When water molecules enter the open osmometer
, they do add volume to the solution, causing the solution level in the osmometer
to rise (graphic).
A 10l.Lm sample of this fluid was analyzed with a vapor pressure osmometer
(Wescor Inc., 5100C, Logan, UT) to estimate tissue osmolality.
We gladly junked unusable glassware, ancient paperwork--not patient records, but outdated procedure manuals and the like--and an antique osmometer
that no longer worked.
Plasma and urine samples were analyzed for concentrations of sodium and potassium ions by flame photometer (Flame photometer 410C, Ciba Corning Inc., USA), chloride ion by Chloridometer (Chloride analyzer 925, Ciba Corning Inc., USA) and osmolality by osmometer
3D3, Advance Instrument Inc., USA).
line includes Vapor Pressure Osmometer
to determine average molecular weight of polymers and osmolality of aqueous solutions; Membrane Osmometer
to measure average molecular weight Mn of polymers; and Cryoscopic Osmometer
to determine molar masses in benzolic solutions.
The osmotic pressures of 10-[[micro]liter] samples were measured in a Wescor Model 5300 vapor pressure osmometer
Osmolality of xylem sap samples and the nutrient solution was determined with a freezing point depression osmometer
(Microosmette 5004, Precision Systems, Inc., Natick, MA).
Osmolarity was determined by using a freezing point osmometer
, osmomat 030, Genotec).
Mesogleal fluid from both bell and tentacles was drawn into calibrated hemocrit tubes and osmotic pressure determined using a Wescor 5500 vapor pressure osmometer
. Osmotic pressure was also measured in the corresponding samples of external media.
Cost of equipment for a tumor immunology lab Approximate Instrument cost Laminal flow hood $ 8,000 [CO.sub.2], water-jacketed incubator 5,000 Water baths, shakers, rockers 1,000 Inverted microscope 4,500 Cryostat, liquid nitrogen tank 12,000 Pipet guns 800 Micropipets, adjustable 500 Beta counter 17,000 Gamma counter 7,000 Cell harvester 5,000 Heater 500 pH meter 500 Osmometer
900 Total $ 62,700