Parts of a gill of this bivalve were removed, washed with seawater, postfixed in 2% osmic
acid in seawater, dehydrated in ethanol, embedded in Spurr resin, sectioned and examined as for the Middle Valley specimens.
Aqueous solutions of "osmium tetroxide," or "osmic
acid," are superior oxidizing agents for organic synthetic reactions.
The system is composed of 3rd generation Osmic
VariMax optics, the Saturn 944 CCD detector, and the AFC-Kappa goniometer.
Carpel walls were rinsed in a cacodilate-sucrose solution and fixed in a 0.1 M cacodilate solution with 1% osmic
tetroxide for 1 to 2 h.
OSMIUM tetroxide also known as osmic
acid is a catalyst used in labs around the world.
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For better identification of nervous tissue, we used an osmic
acid fixation and examined whole preparations and serial semithin sections.
The small tissue blocks fixed in 3% glutaraldehyde were transferred to 1% osmic
acid solution for re-fixation.
The cells were double-fixed by glutaraldehyde and osmic
acid, dehydrated by gradient acetone, immersed in embedding medium, ultrathin-sectioned using an automatic microtome (LeicaRM2235, Leica, Germany), and stained with 1% uranyl acetate.
The samples were fixed in the dark using 1% osmic
acid for 2 h, followed by another three PBS washes for 10 min each.