In Drosophila, the 4E-BP ortholog
, Thor, is upregulated in response to bacterial infection.
This was done by "replacing the key region in the PAM interaction (PI) domain of Staphylococcus aureus Cas9 (SaCas9) with the corresponding region in a panel of SaCas9 orthologs
To identify the potential mouse ortholog
of slincR, we mined an unpublished RNA-seq dataset from male and female mouse E16.75 urogenital epithelial tissue exposed to 5 [micro]g/kg of TCDD, which was generated by the Richard Peterson lab from the University of Wisconsin-Madison.
elegans genes with human orthologs
. PLoS One 2011; 6: e20085, doi: 10.1371/journal.pone.0020085.
DDI2 is an ortholog
of Saccharomyces cerevisiae DNA-damage inducible 1 (Ddi1, also known as Vsm1).
In addition, the Bchs ortholog
gene in Drosophila leads to Rab 11-dependent vesicular trafficking.
To this end, an antibody raised against human Arc was applied to horizontal sections across the entire dorsoventral axis of the adult zebrafish brain, and a putative Arc ortholog
(AIP) was evaluated with Western blot.
The fly genome contains approximately 14,000 genes, and it is estimated that nearly 75% of the genes implicated in human diseases have functional orthologs
in the fly, with 80 to 90% similarity in conserved functional domains at the nucleotide level or protein sequence [104-106].
Recent research has assigned the murine ortholog
caspase-11 guard functions within the gastrointestinal tract in inflammatory states [60-62].
Chen et al., "Using OrthoMCL to assign proteins to OrthoMCL-DB groups or to cluster proteomes into new ortholog
groups," Current Protocols in Bioinformatics, Chapter 6, pp.
detection was done with the Proteinortho software (blastp) with a similarity cut-off of 50% and an E value of 1e - 10.
Genes from the newly annotated genomes and those from the reference strains L550 and JB197 were clustered into ortholog
groups by using OrthoMCL .