Vignette #1: The tRNA tag hypothesis and the origin of replication
The scientists used this technique to label an area near the origin of replication
, a well-kn own site on bacterial chromosomes.
A significant base substitution or insertion in an origin of replication
is an example of such a mutation.
Downstream from the IR, identity was found with a portion of plasmid R751 (positions 9796 to 9947, numbering according to GenBank accession number U67194), located in the vicinity of the oriV vegetative origin of replication