Purification and properties of orcinol hydroxylase from Pseudomonas putida 01.
Metabolism of resorcinylic compounds by bacteria: orcinol pathway in Pseudomonas putida // J.
The RNA content of the 5% PCA extract can be determined using the orcinol reaction (Schneider, 1957).
Orcinol reagent (3.0 ml) is again added to each tube and the samples are heated at 100[degrees]C for 15 minutes.
As measured by the orcinol
assay, only 1% of the mass of the adhesive mucus was carbohydrate.
or [beta]-orcinol derivatives) joined by an ester linkage (i.e.
Kitanaka, "Structures and histamine release inhibitory effects of prenylated orcinol
derivatives from Rhododendron dauricum," Journal of Natural Products, vol.
RNA extract was prepared in 20% cold PCA and estimated according to the Orcinol
method, while DNA was extracted in 10% hot perchloric acid (PCA) and estimated according to diphenylamine method.
* pure compounds including phenol, cresols, resorcinol, orcinol
and higher alkyl mono- and di-hydric phenols
, dipotassium hydrogen phosphate ([K.sub.2]HP[O.sub.4]), n-butanol, potassium dihydrogen phosphate (K[H.sub.2]P[O.sub.4]) and trichloroacetic acid (TCA) were purchased from El-Nasr Chemical Co.
derivatives tenuiorin (1) and methyl orsellinate (2) were identified as active components of an extract from the lichen Peltigera leucophlebia (Nyl.) Gyeln.