orcein


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orcein

 [or-se´in]
a brownish-red coloring substance obtained from orcinol; used as a stain for elastic tissue.
Miller-Keane Encyclopedia and Dictionary of Medicine, Nursing, and Allied Health, Seventh Edition. © 2003 by Saunders, an imprint of Elsevier, Inc. All rights reserved.

or·ce·in

(ōr'sē-in), [old C.I. 1242]
A natural dye derived from orcinol by treatment with air and ammonia; as a purple dye complex it is used in various histologic staining methods.
Farlex Partner Medical Dictionary © Farlex 2012

or·ce·in

(ōr'sē-in)
A natural dye derived from orcinol that is used in various histologic staining methods.
Medical Dictionary for the Health Professions and Nursing © Farlex 2012
References in periodicals archive ?
Advantages and Disadvantages of Orcein, Shiff Reagent, and Hematoxiline-Eosin Use.
In the twentieth century, biologists found orcein to be a useful agent for staining chromosomes, enhancing their visibility under a microscope.
The cell suspension was stained with 5 [micro]L of lacto-acetic orcein, covered with a cover slide and sealed with glue.
It can be demonstrated by rhodamine stain (positive orange granules) or indirectly by orcein stain, which highlights copper-binding protein.
Meristematic regions with 1 mm of length excised and squashed in a drop of 1% acetic orcein mixed with a drop of 45% acetic water (Jahier 1992).
Subsequently the fixed oocytes were stained with 1% orcein. The stage of nuclear maturation was determined as described earlier (Mahmoud 2004).
To prepare the slides, the meristematic regions were covered with coverslips and carefully squashed into a drop of 2% acetic orcein solution.
The samples were then stained with 1% (w/v) orcein in 45% (v/v) acetic acid solution and examined under a phase-contrast microscope (IX-50, Olympus, Tokyo, Japan) at 400x.
Permanent slides were made after softening the tissues in 45% acetic acid, drying on a hot plate and staining with lacto-acetic orcein for 3 to 5 min.