orcein


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orcein

 [or-se´in]
a brownish-red coloring substance obtained from orcinol; used as a stain for elastic tissue.

or·ce·in

(ōr'sē-in), [old C.I. 1242]
A natural dye derived from orcinol by treatment with air and ammonia; as a purple dye complex it is used in various histologic staining methods.

orcein

/or·ce·in/ (or-se´in) a brownish-red coloring substance obtained from orcinol; used as a stain for elastic tissue.

or·ce·in

(ōr'sē-in)
A natural dye derived from orcinol that is used in various histologic staining methods.

orcein

a brownish-red coloring substance obtained from orcinol; used as a stain for elastic tissue.

orcein-Giemsa stain
useful because of its capacity to stain elastic fibers, the metachromatic granules of mast cells, and differentiates smooth muscle from collagen.
References in periodicals archive ?
The orcein staining protocol used in this study was highly reliable for determining chromosome numbers in early embryos.
At 24 [+ or -] 1 h, some DOs in each group were fixed with acetic alcohol and stained with 1% acetic orcein in a manner similar to that used for matured oocytes to assess the rate of sperm penetration.
In the laboratory, permanent slides were made after softening the tissues in 45% acetic acid, drying on a hot plate and staining with lacto-acetic orcein for 3 to 5 min.
The young, germinated radicle apices fixed with acetic alcohol were treated with acetic orcein using the squash technique.
Paraffin section of 10micron thickness were cut with the help of rotary microtome and stained with orcein for elastic fibres and H & E staining for smooth muscle fibres.
After culture, the oocytes were freed from the cumulus cells by vortexing for 2 min, mounted, fixed in 25% (v:v) acetic alcohol for 48 to 72 h at room temperature, and stained with 1% (w:v) orcein in 45% (v:v) acetic acid.
Chromosome number (2n = 4x = 40) was determined by microscopic observation of root tips that had been pretreated for 2 h in a saturated aqueous solution of otbromonaphthalene, transferred to 5 M HCI for 2 min, then stained in basic fuchsin (1%, v/v) for several hours and squashed in acetic orcein (1%, v/v).
Although Rushton bodies shows strong positivity with Papanicolaou stain, Masson's Trichrome, Orcein stains and Prussian blue.
After the removal of cumulus cells, the oocytes were fixed in the fixation solution of 1:3 (acetic acid:methanol) for approximately for 2 minutes, and were stained with 1% orcein (Sigma, USA), and were fixed during the 2 days in acetic alcohol.