Advantages and Disadvantages of Orcein
, Shiff Reagent, and Hematoxiline-Eosin Use.
In the twentieth century, biologists found orcein
to be a useful agent for staining chromosomes, enhancing their visibility under a microscope.
The cell suspension was stained with 5 [micro]L of lacto-acetic orcein
, covered with a cover slide and sealed with glue.
staining was used to quantify the number of elastic fibers in the tunica media, also counting the internal and external elastic membranes.
It can be demonstrated by rhodamine stain (positive orange granules) or indirectly by orcein
stain, which highlights copper-binding protein.
Each slide was stained with two drops of 2% acetic orcein
and examined under an optical microscope at 40X.
Meristematic regions with 1 mm of length excised and squashed in a drop of 1% acetic orcein
mixed with a drop of 45% acetic water (Jahier 1992).
Subsequently the fixed oocytes were stained with 1% orcein
. The stage of nuclear maturation was determined as described earlier (Mahmoud 2004).
To prepare the slides, the meristematic regions were covered with coverslips and carefully squashed into a drop of 2% acetic orcein
The samples were then stained with 1% (w/v) orcein
in 45% (v/v) acetic acid solution and examined under a phase-contrast microscope (IX-50, Olympus, Tokyo, Japan) at 400x.
Oocytes were stained with 1% aceto-orcein (1% orcein
in 45% glacial acetic acid).
Permanent slides were made after softening the tissues in 45% acetic acid, drying on a hot plate and staining with lacto-acetic orcein
for 3 to 5 min.