staining protocol used in this study was highly reliable for determining chromosome numbers in early embryos.
At 24 [+ or -] 1 h, some DOs in each group were fixed with acetic alcohol and stained with 1% acetic orcein
in a manner similar to that used for matured oocytes to assess the rate of sperm penetration.
In the laboratory, permanent slides were made after softening the tissues in 45% acetic acid, drying on a hot plate and staining with lacto-acetic orcein
for 3 to 5 min.
A comparative study of the karyotype with DAPI or acetic orcein
of 5 populations of A.
The young, germinated radicle apices fixed with acetic alcohol were treated with acetic orcein
using the squash technique.
Paraffin section of 10micron thickness were cut with the help of rotary microtome and stained with orcein
for elastic fibres and H & E staining for smooth muscle fibres.
After culture, the oocytes were freed from the cumulus cells by vortexing for 2 min, mounted, fixed in 25% (v:v) acetic alcohol for 48 to 72 h at room temperature, and stained with 1% (w:v) orcein
in 45% (v:v) acetic acid.
0 g of synthetic orcein
to 80 mL of glacial acetic acid in 80 mL of distilled water.
Chromosome number (2n = 4x = 40) was determined by microscopic observation of root tips that had been pretreated for 2 h in a saturated aqueous solution of otbromonaphthalene, transferred to 5 M HCI for 2 min, then stained in basic fuchsin (1%, v/v) for several hours and squashed in acetic orcein
Although Rushton bodies shows strong positivity with Papanicolaou stain, Masson's Trichrome, Orcein
stains and Prussian blue.
Subsequently, the oocytes of each group were stained with 1% acetic orcein
After the removal of cumulus cells, the oocytes were fixed in the fixation solution of 1:3 (acetic acid:methanol) for approximately for 2 minutes, and were stained with 1% orcein
(Sigma, USA), and were fixed during the 2 days in acetic alcohol.