Modulation of spontaneous and evoked EPSCs and IPSCs in optic lobe
neurons of cuttlefish Sepia officinalis by the neuropeptide FMRF-amide.
The OG is located over the optic stalk close to the optic lobe
, it is covered by a fine connective layer, and has an elongate shape.
PARP activity was assayed in optic lobes
, supraesophageal mass, and optic nerves.
1983), we optimized an SV isolation protocol for squid (Logilo pealei [LeSueur, 1821]) optic lobe
in this study to obtain a highly pure and intact SV population for biochemical and ultrastructural studies.
After the animals were sacrificed, the optic lobes
were rapidly dissected from the animals and frozen at -70 [degrees]C.
The cuttlefish optic lobe
comprises an outer granule cell layer, a neurophil layer, an inner granule cell layer (the outer cortex), and numerous clusters of cell bodies separated by areas of nerve fibers and synaptic areas (the central medulla) (5).
Two major parts of the nervous system, the optic lobes
and the nervous system of the arms, lie outside the brain capsule.
We refer to these regions as the dorsofrontal neuropil, optic lobe
neuropil, and median protocerebral neuropil.
Squid optic lobe
(8-[[micro]meter]-thick cryosections) was used as a positive control tissue and gave positive results similar to those of Nelson et al.
In previous experiments we have shown that the synaptosomal fraction from squid optic lobe
Squid optic lobe
membranes (10-75 [[micro]gram] protein) were combined with the indicated concentrations of mono-iodo [125I]-daYFnLRFa, prepared and purified to 2200 Ci/mmole as before (Payza, 1987), in a total volume of 300 [[micro]liter] of 40 mM Pipes-Tris (pH 6.
We have previously shown that a system of protein synthesis is present in the squid giant axon (1, 2) and, more recently, in the nerve endings of the synaptosomal fraction prepared from squid optic lobe