In a separate experiment, typical neutrophil stimulants such as opsonized
zymosan, N-formylmethionyl-leucyl-phenylalanine, and phorbol myristate acetate showed much higher ROS generation in human neutrophils than in whole blood.
Thus, integrins participate in Fc[gamma]R-mediated phagocytosis by promoting adhesion to the opsonized
At the same time, the engulfment percentage of opsonized
and unopsonized SRBCs was also calculated as per formula:
This mechanism inhibits the activation of macrophages and the phagocytosis of opsonized
pathogens and other elements that are susceptible to recognition by IgG-class antibodies.
The oxidative burst in whole blood was stimulated with phorbol myristate acetate (PMA 0.05 [micro]M), opsonized
zymosan (OpZ; 0.5mg/mL), fMLP (1 [micro]M), or Ca ionophore A23187 (1 [micro]M).
Carneiro-Sampaio, "Colostral mononuclear phagocytes are able to kill enteropathogenic Escherichia coli opsonized
with colostral IgA," Scandinavian Journal of Immunology, vol.
blazei structures, opsonized
with human serum in vitro.
In the oxidative burst activity experiments, upon stimulation with unlabeled opsonized
Sheep red blood cells (RBCs) were opsonized
with a subagglutinating concentration of IgG rabbit antisheep erythrocyte antibody (Cappel Organon Teknika, Durham, NC) as previously described .
Another important activating receptor is represented by CD16 (or Fc[gamma]RIIIA), the low affinity receptor for immunoglobulin G (IgG), able to bind immune complexes and IgG-coated, opsonized
paradoxa extract on the oxidative burst of neutrophils and monocytes induced by various stimuli, including opsonized
After addition of 10 [micro]l opsonized
zymosan, the measurement was initiated and finished after 60 min.