Maximum-likelihood phylogenetic analyses were performed by using full-length open reading frame
nucleotide sequences of V585 and other group A rotavirus strains (online Technical Appendix).
In line with our data, nucleotide (and amino acid) sequence identities of 50%-53% (42%-49%), 51% 57% (42%-55%), and 45%-46% (20%-29%) were reported for open reading frame
(ORF) 1, ORF-2, and ORF-3, respectively, between rat, human, and avian HEV strains (1).
In case-patient 1 reported here, HEV sequences recovered from serum and CSF were 100% identical; nevertheless, they were compared on a short open reading frame
2 fragment (106 nt) and on samples collected at time of acute hepatitis E, whereas comparison was performed 33 months postacute hepatitis in the case reported by Kamar et al.
Translational attenuation occurs when the translation of one open reading frame
alleviates inhibitory secondary structures in a downstream open reading frame
Invitrogen is a leading provider of recombinant cloning and protein expression products, as well as the premier provider of the largest fully sequenced human open reading frame
The goal of the MGC project, a trans-NIH initiative co-managed by the National Human Genome Research Institute (NHGRI) and the National Cancer Institute (NCI), is to identify and sequence representative full open reading frame
(ORF) cDNA clones for all human and mouse genes, as well as a subset of rat genes.
With the open reading frame
1, spike, envelope, membrane, and nucleocapsid gene segments and NS7 accessory gene phylogenetic trees, the US PDCoV strains clustered separately from the China PDCoV strains (Figure, panel B).
From building open reading frame
clone collections and cDNA libraries, to protein detection, purification and production, Gateway(R) plays a vital role.
The open reading frame
(ORF) 1a gene was used for confirmation as recommended by the World Health Organization(www.
announces the release of over 8,000 unique Human open reading frame
(ORF) clones from full-length genes.
It was very surprising and exciting to discover that ARFs can be better immunogens and targets than the protein derived from the main open reading frame
of a gene.
contigs Range of matching contig lengths, Alphacoronavirus gene alphacoronaviruses bp Open reading frame
lab 33 182-1054 Spike protein 5 580-1629 Matrix 4 251-840 Nucleocapsid 4 536-890 Highest scoring BLASTn hit recorded against Alphacoronavirus gene alphacoronaviruses e-value Nucleotide identity (%) ([dagger]) Open reading frame
lab 8 x [10.