open reading frame

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o·pen read·ing frame

a gene presumed to code for a protein but for which no gene product has been identified; also known as unidentified reading frame.

open reading frame

In RNA, a sequence of base pair triplets (codons) with no introns, which is translatable into a protein.

open reading frame (ORF)

a sequence of NUCLEOTIDE bases between an INITIATION CODON and a TERMINATION CODON, with the potential to be translated into a POLYPEPTIDE. Not all ORFs, especially the short ones, correspond to genes.
References in periodicals archive ?
The open reading frame encoded a 255 amino acid residue protein with an isoelectric point (pI) of 5.
Read mappings from the (A) Templiphi-generated muscle library for the coI open reading frame, (B) nd4 open reading frame, (C) D-loop, and (D) nd6 open reading frame.
2 noroviruses in China, reconstructed based on RNA-dependent RNA polymerase (A) and open reading frame 2 (B) with a representative norovirus using the neighbor-joining method with datasets of 1,000 replicates in MEGA 6.
The open reading frame encoded a 157 amino acid residue protein with an isoelectric point (pI) of 7.
Contract awarded for Constitutive expression in mammalian cell lines of the complete open reading frame of a specified protein tagged at C-terminal with green fluorescent protein (GFP).
For the temporal and spatial expression study, the promoters for flhD (flagellar master regulator) and ompR (osmoregulator) were fused in front of the open reading frame for green fluorescence protein (GFP).
Additionally, we have sequenced the entire mtMSH gene for multiple octocorals and preliminary analysis shows that this gene has an open reading frame in all species.
If a 5'-proximal AUG triplet can serve as a sufficient translational initiation signal, then any AUG sequence positioned in this manner might conceivably be translated, mRNA conformation permitting, regardless of whether or not the utilized start codon is part of a legitimate open reading frame ending with one of the three possible termination codons.
We used a nested RT-PCR to amplify a 345-nt sequence within HEV open reading frame 2 as described (8).