In mature oocytes, the nucleus was dissolved and the ooplasm
which consists of yolk bodies were also monitored (Fig.
Vacuoles were dispersed in the ooplasm
forming two distinct rings, one around and near the nucleus while the other near the periphery of the oocyte.
transfer is a technique to enhance fertility, not to specifically diagnose or modify the embryo.
Hence, in this study, mitochondria-specific fluorescent probes (Mito Traker Green) were used to show the distribution of mitochondria within the ooplasm
An inadvertent consequence of this procedure was that mitochondrial DNA found in the ooplasm
of the donated material was introduced into the recipient eggs.
Thereafter, calcium transients spread across the entire ooplasm
as point-source waves and usually attain lower amplitudes than are exhibited by the calcium waves of mammalian fertilizations (Stricker, 1999).
Observation revealed that monocrotophos mainly affected earlier stages of development in testes and ovaries of Chrotogonus trachypterus, which were severely damaged after a prolonged time, exhibiting contraction of ooplasm
and nuclear membrane (Shakeet & Bakshi 2009).
Oocytes of quality I and II, with compact cumulus mass and uniform ooplasm
were morphologically selected according to De Loos et al.
Oocytes were graded by the morphological appearance of the cumulus cells and ooplasm
under zoom stereomicroscope (Chauhan et al.
On the other hand, another limit was identified microscopically at 1 mm, corresponding to the smaller size at which yolk was present in the ooplasm
The zygote centrosome has duplicated centrioles and maternal gamma tubulin is further added to the PCM in the ooplasm
to make it a functional centrosome, when the centrosome forms the sperm monoaster.
Oocytes of primordial follicles were enclosed by simple cubic epithelium (pregranulosa cells), showing eccentric nuclei and acidophilic cytoplasm containing small vacuoles in the peripheral region of the ooplasm