Amongst the post-Matric qualified husbands 13 (39%) were in the 2 offspring category, 12 had 3 offsprings each, 5 had 4 offsprings each and only 3 were in the 5 offsprings category.
Percentage distribution of the number of offsprings in the three educational categories of group A and B reveals that lower the education status, more is the number of offsprings in both men and women in this study group.
After categorizing the subjects of the present study according to the level of education achieved at the time of conducting the study, it was evident from the findings, that in each group there is a decline in the total number of offsprings as the educational status rose up.
Anxiety-like behavior of male offspring was assessed by performing elevated plus maze and open field tests (Bailey et al.
Male and female offspring at 12 weeks of age, including the offspring used previously to test behavioral analyses, were individually caged and fasted (water was provided ad libitum) for 6 hr in the morning before initiation of the experiment (n = 15 males and n = 14 females per treatment).
A second experiment was conducted as described above to allow for assessment of additional indicators of metabolic syndrome (described below) in male offspring of treated and control dams.
Thirteen-week-old male offspring of control and treated females were individually caged and fasted (water was provided ad libitum) for 6 hr in the morning before starting the experiment (n = 34 for controls and n = 35 for treated offspring; two offspring in control and one offspring in treatment group were excluded from the test due to signs of excessive stress at initiation of experiment).
Experiment 2 was terminated at two different time points when male offspring were 13 and 25 weeks of age.
Blood samples were obtained from offspring when Experiment 2 was terminated at 25 weeks of age (n = 19 for controls and n = 17 for treated offspring).
Likewise, in the liver, transcript abundance for genes required for fatty acid uptake (FATP2 and CD36) and glucose homeostasis (G6PC) was analyzed in male offspring of control and treated females.
Total RNA was isolated from adipose and liver tissue of male offspring using an RNeasy[R] Mini kit (Qiagen, Valencia, CA, USA) based on the manufacturer's instructions.
Initial tests showed that transcript abundance for HPRT in adipose and liver tissue was similar between control and treatment group offspring (p > 0.