A reference line was marked at 2 mm from the central occlusal fissure
using a diamond disc (MDT Micro Diamond, Afula, Israel) mounted on a low-speed handpiece (MTI, New Jersey, USA) with water irrigation in order to delimit the area to be treated.
When evaluating occlusal fissure
areas in the SOPROLIFE blue fluorescence mode, we used the SOPROLIFE blue fluorescence mode score description as presented in Table 2.
Teeth with occlusal restorations, occlusal fissure
sealants, extensive fissure staining, hypoplastic pits and occlusal caries with cavitation were excluded from the sample.
The histological presence of caries was determined in bucco-lingual serial sections ([approximately equal to]1 mm thick) sectioned from the occlusal fissure
system (Minitom, Streurs, Copenhagen, Denmark).
Penetration of acid solution and gel in occlusal fissures
. J Am Dent Assoc 1987; 114: 809-810.
Hence, appropriate intervention therapy should be provided to high caries risk children and this include appropriate fluoride therapy, fissure sealant for their non-carious occlusal fissures
, minimal intervention therapy for the cavitated lesions, and diet and plaque control counselling.
A study carried out with human molars (sound and with visible carious lesion in fissures) mapped the distribution of fluoride and calcium in the surface enamel of the occlusal fissures
and determined the association between variations in fluoride concentration and the occurrence of demineralised areas in the enamel and subjacent dentine.
Er:YAG laser effects on enamel occlusal fissures
: An in vitro study.
 reported that FS after 15 and 20 years showed 40% of the failed FS on the mandibular first molar had failed because of caries or restoration in the buccal pits, while the FS in the occlusal fissures
showed complete retention.