BACTERIAL ACIDIFICATION AND CAVISTAT ALKALINIZATION OF OCCLUSAL FISSURE pH
Earlier, we showed with a 2 micron diameter tip Sb electrode that oral bacteria cause occlusal fissure pH to become and remain highly acidic for long hours after carbohydrate (CHO) exposure.
Teeth with occlusal restorations, occlusal fissure
sealants, extensive fissure staining, hypoplastic pits and occlusal caries with cavitation were excluded from the sample.
The histological presence of caries was determined in bucco-lingual serial sections ([approximately equal to]1 mm thick) sectioned from the occlusal fissure
system (Minitom, Streurs, Copenhagen, Denmark).
A study carried out with human molars (sound and with visible carious lesion in fissures) mapped the distribution of fluoride and calcium in the surface enamel of the occlusal fissures
and determined the association between variations in fluoride concentration and the occurrence of demineralised areas in the enamel and subjacent dentine.
2001] reported that FS after 15 and 20 years showed 40% of the failed FS on the mandibular first molar had failed because of caries or restoration in the buccal pits, while the FS in the occlusal fissures
showed complete retention.
2003] tested in vitro the ultrastructure of resin-enamel bonds in occlusal fissures
with unground enamel using phosphoric acid etching in combination with a FS and two single-step self-etch adhesives (Adper Prompt-L-Pop[R], 3M Espe and Xeno III[R], Dentsply De Trey] followed by a FS.
Recently, a different morphology of occlusal fissures
has been described [Kidd et al.