MINAMIGUCHI and MACHADO NETO (2007) did not obtain regenerated plants from the somatic embryos formed in their study, since these embryos, and the nutrient medium
, underwent phenolization; it may have happened because the embryos were kept in the induction medium containing ANA and BAP.
As a result, a growth of mycobacterium in the form of a cloud or haze in the nutrient medium
was detected on the fifth day in test tubes with the DIR medium (Figure 5).
In the next step of screening, [gamma]1 and [delta]1 could not grow on synthetic dropout nutrient medium
(SD/-Leu/Trp/Aba/X-[alpha]-Gal [DAX]), suggesting that a single GAL4AD-Rheb/FKBP38 does not activate downstream reporter genes; thus, GAL4AD-Rheb and GAL4ADFKBP38 could be used for further screening of yeast twohybrid assay as prey protein.
The results show the existence of significant differences in the average concentration of alkaloids in the stalks by the differences in the added concentration of Auxins and Cytokinins in the nutrient medium
(F test significance value [less than or equal to] 0.05).
(3) 50 mL of nutrient medium
is prepared and sterilized.
was cultivated on Bogdanov's modified nutrient medium
with the 2 times increased maintenance of nitric components.
As a nutrient medium
, again, CP medium with 30 g [L.sup.-1] sucrose, 0.08 mg [L.sup.-1] Vitamin B12, 0.02 mg [L.sup.-1] IAA and E20A medium without agar were used.
Next, first half of the samples was poured over with the nonfully valuable nutrient medium
inoculated with bacteria--[10.sup.6] cells/mL (polish standard: PN-EN ISO 846:2002) and a second half was poured over with the nutrient medium
without bacteria (control samples).
Any nutrient medium
and energy source that supports the growth of Campylobacter may be used.
The 55-Plus monitor base features a gridded microfiltration-type membrane bonded to an absorbent pad, which prevents the membrane from bowing when nutrient medium
We have described the development of a soy based nutrient medium
and have demonstrated that its usefulness in typical microbiology experiments is comparable to that of conventional, animal-based media.
Short-term storage in liquid nutrient medium
is clinically desirable for certain length of time.