Furthermore, one locus had relatively high estimate of null allele
frequency and Hardy-Weinberg equilibrium was not possible to calculate for other 3 loci.
The molecular basis of a microsatellite null allele
from the White Sands pupfish.
e]) heterozygosity, estimated frequencies of null alleles
(r) and polymorphic information content (PIC) for the eight SSR loci are shown in Table 4.
A sample of the apparent TPOX null allele
carriers who were identified in the way described above were re-profiled using the COfiler or Identifiler kits, which use different primers to those employed in the Powerplex 16 kit.
2007: Inference of population structure using multilocus genotype data: dominant markers and null alleles
Some of these loci have exhibited strong evidence of null alleles
In the context of a breeding population, the effect of a null allele
is to mistakenly reject a true father, due to a lack of observed shared alleles with the offspring; that is, the number of real parents is underestimated.
We note that our sample sizes were small for all 6 populations (N = 10); larger samples of individuals would have provided a more robust estimate of the rate of selling and null allele
We removed three microsatellite loci (MSMM5, MSMM8, and Moe7) from final analyses because they showed evidence for a null allele
If evidence was found for a null allele
in any sample and locus combination, the frequency of the null allele
was calculated as the average of the estimates from 3 independent methods (Chakraborty et al.
Among these, 11 that showed clear PCR products without the indication of a null allele
were selected, which are listed in Table 1.
A null allele
was assigned if either no amplicon was detected or agarose gel electrophoresis data showed an amplicon of a size that was beyond the usual range of fragment size of the specific VNTR locus.