Our phylogenetic analysis of the eubacterial, eukaryal, and archaeal CCA-adding enzymes shows that all known CCA-adding enzymes do indeed belong to a single nucleotidyltransferase superfamily, as might be expected if this activity was present in the last common ancestor of contemporary organisms.
Isolation of a temperature-sensitive mutant with an altered tRNA nucleotidyltransferase and cloning of the gene encoding tRNA nucleotidyltransferase in the yeast Saccharomyces cerevisiae.
tRNA nucleotidyltransferase is not essential for Escherichia coli viability.
Taq polymerase; T4 polynucleotide kinase; terminal deoxy nucleotidyltransferase
(tdT); T7 RNA polymerase; and SP6 RNA polymerase.
aminoglycoside acetyltransferases (AACs), aminoglycoside nucleotidyltransferases
(ANTs), and aminoglycoside phosphotransferases (APHs), each of which will chemically convert one or more substituents of the aminoglycosides (Fig.
These enzymes, classified as aminoglycoside acetyltransferases, nucleotidyltransferases
or phosphotransferases, catalyze the transfer of acetyl, adenosine monophosphate or phosphate groups onto the aminoglycoside antibiotics (Wright et al.