Lingenfelter BM, Tripurani SK, Tejomurtula J, Smith GW, Yao J (2011) Molecular cloning and expression of bovine nucleoplasmin
2 (NPM2): a maternal effect gene regulated by miR-181a.
It was very elegantly demonstrated that karyophilic proteins require a signal to reach the nucleus as their destination using nucleoplasmin, a nuclear protein from Xenopus laevis, which functions as a chaperone for nucleosome formation.
cNLSs are divided into two types, a monopartite type such as the NLS of the SV40 large T antigen (PKKKRKV) consisting of one cluster of basic amino acids, and a bipartite type such as that of nucleoplasmin (KRPAATKKAGQAKKKK) composed of two clusters of basic amino acids separated by linker amino acids.
Typical monopartite cNLSs, such as the SV40 large T antigen NLS, bind to the major binding site, whereas bipartite cNLSs, such as the nucleoplasmin NLS, bind to both the major and minor binding sites.
Banna Mini-pig Inbred Line, Prokaryotic expression, Nucleoplasmin
, Polyglutamic acid, Embryo development.
Another study by Shen and colleagues, with 317 MDS patients, demonstrated that CDH1; CHD13; ER[alpha]; NOR (oxidored-nitro domain-containing protein isoform 1), a gene that encodes two transcripts and acts as a tumor suppressor; NPM2 (nucleoplasmin
2), involved in chromatin reprogramming; OLIG2 (oligodentrocyte lineage transcription factor 2), involved in the chromosomal translocation t(14;21)(q11.2;q22) which is associated with T-cell acute lymphoblastic leukemia; CDNK2B; PGRA (progesterone receptor A), which functions as transcriptional activator or repressor; and RIL (PDZ and LIM domain 4), localized in a region frequently deleted in AML and MDS, were methylated in MDS/AML patients.
Aoki, "Involvement of mouse nucleoplasmin
2 in the decondensation of sperm chromatin after fertilization," Biology of Reproduction, vol.
The monopartite NLS prototype is the SV40 large T antigen (126PKKKRRV132), characterized by one short basic stretch containing 4-8 lysine and arginine residues, and the bipartite NLS has an NLS motif (155[KRP.bar]AATKKAGQA[KKKK.bar]170) that was originally in the Xenopus protein nucleoplasmin
This mutant and other members of the nucleoplasmin
family, which lack the C-terminal nucleic acid binding region, B23.2 and NPM3, showed a greater mobility/faster recovery than the B23.1, indicating the importance of this segment.
Nucleoplasmin (Npm) is the most abundant protein in the nucleus of oocytes which can promote in vitro nucleosome formation.
Nucleoplasmin (Npm), as a molecular chaperone, was found in the eggs of Xenopus laevis which facilitated sperm chromatin remodeling at fertilization (Leno et al., 1993).
Multiple positive clones were found to contain a cDNA for protein NPM3, which belongs to the nucleoplasmin
family of which protein B23 is also a member.