The characteristic cytomorphologic features noted correspond to the histologic features and include large polygonal to round cells with granular to flocculent cytoplasm, well defined cell borders, mild nuclear pleomorphism, vesicular nuclei, perinuclear clearing, and small to indistinct nucleoli
Loss of the rough endoplasmic reticulum, ribosomes, and nucleoli
apparently inhibits cellular protein synthesis and polymerization of RNA and DNA in heat-treated tumor cells (Bowler et al.
total number of germ cells/ Sertoli cell nucleoli
, to obtain the total support capacity of each Sertoli cell.
2 Photomicrographs show a tumour composed of variably sized tubules (a: Hematoxylin and eosin stain [H&E] x100) and cystic spaces (b: H&E x100) that are separated by thin, hypocellular fibrovascular septae (c: H&E x200), with the lining epithelium showing tombstone appearance focally (d: H&E x200); the tubules are lined by atypical cuboidal to pseudostratified ciliated columnar cells (arrow) with abundant eosinophilic cytoplasm (e: H&E x400), large round to ovoid nuclei with prominent nucleoli
(f: H&E x400) and hobnail appearance (g: H&E x600); solid areas show smaller tubules lined by similar atypical cells in a sclerotic stroma (h: H&E x200; i: H&E x400).
Histologically, all tumors contained noncohesive cells with eccentric vesicular nuclei, prominent nucleoli
, and fibrillar, eosinophilic cytoplasmic inclusions.
Our case showed a mixed population of spindle cells (predominantly) and epithelioid/plasmacytoid cells, demonstrating nuclear pleomorphism, coarse and clumped chromatin, variable inconspicuous to multiple prominent small nucleoli
, and occasionally bi-nucleated tumour giant cells/ bizarre nuclei; and lacks the main cytological features elaborated above of conventional melanoma.
Cells became more scattered after 48 hours and remained round with apparent nucleoli
after 72 hours.
In contrast to ILC cells, PLC cells display a greater degree of cellular pleomorphism, have abundant eosinophilic cytoplasm, greater mitotic activity, and nuclear abnormalities such as hyperchromasia, nuclear membrane irregularities, and prominent nucleoli
(Figure 1, A through D Figure 2, A).
Neoplastic cells had indistinct cell borders; moderate amounts of pale eosinophilic cytoplasm; large, central, oval to round vesicular nuclei with finely stippled chromatin; and multiple, prominent nucleoli
On the other hand, the subcortical half of the biopsy was occupied by large aggregates of monotonous bland-appearing large cells which contained mainly clear and rarely eosinophilic foamy cytoplasm, distinct cytoplasmic membrane, medium-sized, and round eccentric nuclei with chromatin clumping and inconspicuous nucleoli
Viability was indicated by the presence of intact nucleoli
within the cartilage lacunae.
One of the core biopsies showed infiltrative, well-formed, small acini with enlarged nuclei with visible nucleoli
and atrophic cytoplasm (Figure 1, A through C).