It was concluded that the tubular structures initiated by organizing around the icosahedral nucleocapsid
and depending on the number of furin processed proteins incorporated, terminated the envelopment process by normal membrane fission (budding) or by repeated incorporation of hexagonal protein arrays.
Razanskiene et al., "Development of novel immunoglobulin G (IgG), IgA, and IgM enzyme immunoassays based on recombinant Puumala and Dobrava hantavirus nucleocapsid
proteins," Clinical and Vaccine Immunology, vol.
Study of protection by recombinant fowl poxvirus expressing C-terminal nucleocapsid
protein of infectious bronchitis virus against challenge.
Identification of nucleocapsid
binding sites within coronavirus-defective genomes.
Identification of a common antigenic site in the nucleocapsid
protein of European and North American isolates of porcine reproductive and respiratory syndrome virus.
Antigenicity of hantavirus nucleocapsid
protein expressed in E.
(3) Nonstandard abbreviations: hCG, human chorionic gonadotropin; NP, influenza A nucleocapsid
protein; COPS, 3-glycidoxypropyldimethyletho)cysilane; PFU, plaque-forming units; NHS-LC-biotin, sulfosuccinimidyl-6-(biotinamido) hexanoate; SSC, standard saline citrate; PBS, phosphate-buffered saline; and BSA, bovine serum albumin.
Phylogenetic tree based on complete nucleocapsid
gene sequences of Puumala virus (PUUV) strains from Lithuania (LT), Latvia (Jelgava1), and other PUUV clades.
Cloning and expression of Rift Valley fever virus nucleocapsid
(N) protein and evaluation of a N-protein based indirect ELISA for the detection of specific IgG and IgM antibodies in domestic ruminants.
All samples were tested for virus by real-time RT-PCR (5.6) and tested for antibodies against GOLV and HEBV nucleocapsid
proteins by recombinant immunofluorescence assay (10).
Respiratory specimens from the 2 infected humans and the 5 dromedaries that were still positive at the second sampling were analyzed by dideoxy sequencing as previously described (13); the nucleocapsid
gene sequences of all dromedary samples were found to be identical.
The translated nucleocapsid
and S segment nonstructural proteins of both viruses are identical, and the polymerases and glycoproteins share 99% identity.