The tubes were centrifuged at 1,700 x g for 10 min at 4[degrees]C to separate plasma, nucleated cells (including leukocytes and nucleated RBC
in cord blood and leukocytes in maternal blood), and RBC within 24 h after collection.
Peripheral smear picture: RBC are Normocytic, Macrocytic, and Normochromic, Nucleated RBC
5/100, a few polychromatophils are seen, WBC showing over whelming, leukocytosis with shift to left.
Histological examination is the main tool in the diagnosis of molar pregnancy including the degree of trophoblastic hyperplasia, villous contours and scalloping, presence of distinct cisterns, trophoblastic inclusions, and presence or absence of nucleated RBC
in fetal vessels.
Half of the neonates in the seizure group had nucleated RBC
counts that were above 10 per 100 white blood cells, which is considered the upper level of normal, Dr.
4% reliability of the flags for immature granulocytes or left shift, 57% for nucleated RBC
and atypical lymphocytes, and 80% for blast cells.
Another technological advance was the use of fluorescence by a nuclear stain for identifying and enumerating nucleated RBC
, apart from WBC.
In all cases Red blood cell (RBC) morphology showed anisopoikilocytosis, polychromasia, target cells, fragmented RBC, microcytic/hypochromic blood picture, and nucleated RBC
ranging from 2 to 16 /100 WBC(Table 2).
2) Cord blood sample was taken & assessed for Creatine Kinase and Nucleated RBC
At the first station, the digital imaging is performed at low magnification to count RBCs, WBCs, platelets, and nucleated RBCs
rarely seen nucleated RBCS, normal electrophoretic mobility and alkali resistance in Hb.
A confounding factor may be the presence of avian nucleated RBCs
that might reduce the diffusion of glucose from blood samples into the chemical layer of the test strip, thus resulting in the underestimation of blood glucose concentrations.
For unusual WBCs and nucleated RBCs
, sensitivity and specificity were 100% and 97% respectively.