nuclease


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nuclease

 [noo´kle-ās]
any of a group of enzymes that split nucleic acids into nucleotides and other products.

nu·cle·ase

(nū'klē-ās),
General term for enzymes that catalyze the hydrolysis of nucleic acid into nucleotides or oligonucleotides by cleaving phosphodiester linkages. For nucleases not listed here, see the specific term. Compare: exonuclease, endonuclease.

nuclease

/nu·cle·ase/ (noo´kle-ās) any of a group of enzymes that split nucleic acids into nucleotides and other products.

nuclease

(no͞o′klē-ās′, -āz′, nyo͞o′-)
n.
Any of several enzymes, including the endonucleases and the exonucleases, that hydrolyze bonds between nucleotides in nucleic acids.

nuclease

An enzyme that catalyses the hydrolysis of the phosphodiester bond of polynucleotide chains and phosphate-deoxyribose bonds within (endonuclease) or at the end (exonuclease) of a nucleotide sequence (nucleic acid). Nucleases are produced by most biologic systems and usually recognise a specific substrate, such as single- or double-stranded DNA or RNA.

nu·cle·ase

(nū'klē-ās)
General term for enzymes that catalyze the hydrolysis of nucleic acid into nucleotides or oligonucleotides.
Compare: exonuclease, endonuclease

nuclease

Any one of several enzymes that break down NUCLEIC ACIDS.

nuclease

any enzyme that promotes hydrolysis of NUCLEIC ACIDS. For example, DNase, which catalyses the breakdown of DNA into individual DNA NUCLEOTIDES. The enzyme cleaves PHOSPHODIESTER BONDS of the nucleic acid. See ENDONUCLEASE, EXONUCLEASE.

nuclease

any of a group of enzymes that cleave or digest nucleic acids into fragments or single nucleotides.

S1 nuclease
a single-strand-specific endonuclease that degrades DNA and RNA to nucleoside 5′-monophosphates. The enzyme is a single polypeptide chain of molecular weight 32,000 and is isolated from 'takadiesterase', a digestive enzyme preparation from Aspergillus oryzae. Widely used in molecular biology to map the position of mRNA to its DNA, and also used to remove single-stranded tails from DNA fragments, to produce blunt ends to open up hairpin-loop structures.
References in periodicals archive ?
These combined features significantly boost signal-to-noise ratios compared with traditional 5' nuclease PCR assays (Figures 3A, 3B) and deliver calls with >99.
During specificity, heat treatment at 80 degC for 20 minutes of soil DNA sample prior PCR improved detection level and nuclease enzymes became inactivated.
Newly designated nuclease is termed as Cas protein of PreFran subtype (cpf1) which shows proficient genome editing by targeting PAM (T-rich).
An improved zinc-finger nuclease architecture for highly specific genome editing.
Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases.
The use of pBR322 plasmid DNA as a substratum showed that the extract of granules in patients exhibits nuclease activity which is specific for cleavage of single-strands, since circular form in double strands (II) was stable enough to its action, and was not sensitive to [Zn.
22 [micro]m screen filter (to retain particles and bacteria) or an ultrafilter (to retain nucleases and other bacteria by-products) for sequencing work.
Uropathogenic specific protein gene, highly distributed in extraintestinal uropathogenic Escherichia coli, encodes a new member of H-N-H nuclease superfamily.
The disabling of the CCR5 gene by the nuclease, as well as the addition of the anti-HIV genes, created multiple layers of protection.
This enzyme is a non-specific DNA/RNA nuclease and belongs to a family of nucleases denominated PPa-mefinger, a name based on the disposition of the catalytic site, which comprises two beta sheets and an alpha helix (13).
Inhibition of Ape1 Nuclease Activities by Lead, Iron, and Cadmium
The 5' nuclease assay (TagMan[R]) uses fluorogenic allele-specific detection probes that allow PCR amplification and allele detection in a single procedure (11).