nuclease


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nuclease

 [noo´kle-ās]
any of a group of enzymes that split nucleic acids into nucleotides and other products.

nu·cle·ase

(nū'klē-ās),
General term for enzymes that catalyze the hydrolysis of nucleic acid into nucleotides or oligonucleotides by cleaving phosphodiester linkages. For nucleases not listed here, see the specific term. Compare: exonuclease, endonuclease.

nuclease

(no͞o′klē-ās′, -āz′, nyo͞o′-)
n.
Any of several enzymes, including the endonucleases and the exonucleases, that hydrolyze bonds between nucleotides in nucleic acids.

nuclease

An enzyme that catalyses the hydrolysis of the phosphodiester bond of polynucleotide chains and phosphate-deoxyribose bonds within (endonuclease) or at the end (exonuclease) of a nucleotide sequence (nucleic acid). Nucleases are produced by most biologic systems and usually recognise a specific substrate, such as single- or double-stranded DNA or RNA.

nu·cle·ase

(nū'klē-ās)
General term for enzymes that catalyze the hydrolysis of nucleic acid into nucleotides or oligonucleotides.
Compare: exonuclease, endonuclease

nuclease

Any one of several enzymes that break down NUCLEIC ACIDS.

nuclease

any enzyme that promotes hydrolysis of NUCLEIC ACIDS. For example, DNase, which catalyses the breakdown of DNA into individual DNA NUCLEOTIDES. The enzyme cleaves PHOSPHODIESTER BONDS of the nucleic acid. See ENDONUCLEASE, EXONUCLEASE.
References in periodicals archive ?
Recombinases, nucleases, and accessory proteins are natural or artificially created tools for gene editing that are used for developing powerful methods of genetic engineering.
They found out that approximately all archaeal genomes, as well as CRISPR nucleases, were highly diverse.
Targeted approach to address this challenge was the design of chimeric nucleases which are complex proteins containing one or two structural units, one of which catalyzes the cleavage of DNA, and the second is capable of selectively binding to specific nucleotide sequences of target molecule, providing the nuclease action to this site (Table 1) [46, 47].
HNH nuclease is reported to be involved in cleavage of complementary strand while Ruvc cleaves the non-complementary strand resulting in the blunt end formation which after re-union gives frame shift mutations or indels [9]
Nuclease activity can be quantified by the proportion of digested and undigested PCR products following the formula:
In our study, it was showed that in absence of high quality DNA extraction kit, improvement in detection of FT can be carried out with simple heat inactivation of nuclease enzymes present in environmental samples or patient serum.
Methylation-sensitive enrichment of minor DNA alleles using a double-strand DNA-specific nuclease. Nucleic Acids Res 2016; 45:e39.
It seems therefore reasonable that for genetic cardiac diseases, the in vivo editing approach involving the direct injection of the nuclease system might be a better method.
(b) Fusion of catalytically "dead" Cas9 with dimerization-dependent FokI nuclease domains.
Tandem overproduction and characterisation of the nuclease domain of colicin E9 and its cognate inhibitor protein Im9.
An additional filter may be attached at the outlet of the water purification system, such as a 0.22 [micro]m screen filter (to retain particles and bacteria) or an ultrafilter (to retain nucleases and other bacteria by-products) for sequencing work.
This enzyme is a non-specific DNA/RNA nuclease and belongs to a family of nucleases denominated PPa-mefinger, a name based on the disposition of the catalytic site, which comprises two beta sheets and an alpha helix (13).