Effect of exogenous Npm or PGA on the developmental competence of somatic cell nuclear transfer
embryos in BMI pig.
Specifically, the scientists found that the DNA methylation and gene expression patterns in nuclear transfer
ES cells more closely resembled those of ES cells than did iPS cells, which revealed alterations apparently caused by the reprogramming process itself.
The work "is certainly impressive," says developmental biologist John Gurdon, who shared the 2012 Nobel Prize in physiology or medicine for pioneering the nuclear transfer
technique to clone a frog.
Impeding Xist Expression from the Active X Chromosome Improves Mouse Somatic Cell Nuclear Transfer
has made it known that he believes Missouri should take the lead in outlawing somatic cell nuclear transfer
, regardless of what good may come of the research or the jobs or money the effort brings to the state.
had already been used to produce sheep carrying the human gene for Factor IX, a protein used in the treatment of haemophilia.
Thus, under current law, the use of somatic cell nuclear transfer
to create an embryo solely for research purposes is already restricted in cases involving federal funds.
The aim of this study was to investigate the effects of donor cell passage, size and type on the development of nuclear transfer
This patent family includes granted claims related to somatic cell nuclear transfer
and the use of oocytes and oocyte extracts to reprogram somatic cells.
The nuclear transfer
results in transplanting an animal's genes into an egg.
Examples of research topics that could be addressed under this program announcement are 1) strategies for culturing pluripotent rat ESCs to allow genetic manipulation and to create rats with germ-line transmission of genetic modifications, 2) development of alternative technologies to create null mutations or gene replacement in the rat, 3) development of cost-effective nuclear transfer
procedures in the rat, 4) studies that demonstrate mutation transfer to rat stem cells or other cells for transfer into embryos or germ cells, and 5) methods for targeting engineered introns into rat chromosomal DNA to support the study of gene function.