In an MGH study published in 2018 in the journal Neuron, researchers found that in a brain with Alzheimer's neurons have faulty nuclear pores
, the structures that the cell's nucleus use to communicate with the rest of the cell.
After biochemical purification of the cytosolic extract, we isolated a stable complex called the nuclear pore-targeting complex (PTAC) containing the NLS-substrate to target the nuclear pore
and found that the complex contains two essential components, a 58-kDa protein called PTAC58 and a 97-kDa protein called PTAC97.
The triple A syndrome is due to mutations in ALADIN, A novel member of the nuclear pore
GAS, IFN gamma activated sequence; H3, histone H3; ISRE, IFN sensitive response element; NPC, nuclear pore
Forbes, "Analysis of nuclear reconstitution, nuclear envelope assembly, and nuclear pore
assembly using Xenopus in vitro assays," Methods in Cell Biology, vol.
Age-dependent deterioration of nuclear pore
complexes causes a loss of nuclear integrity in postmitotic cells.
Nuclear poly(A)-binding protein PABN1 is associated with RNA polymerase II during transcription and accompanies the released transcript to the nuclear pore
. Exp Cell Res 2003;286(2):332-344.
"Our results also suggest that nuclear pore
deterioration might be a general aging mechanism leading to age-related defects in nuclear function, such as the loss of youthful gene expression programs."
Papers address nuclear pore
complex organization and nucleoporin functions, structural analysis of karyopherin-mediated nucleocytoplasmic transport, the translocation channel of the nuclear pore
complex, nuclear export of ribosomes, nuclear-cytoplasmic tRNA trafficking, mRNA export as an integrative component of gene expression, import and export of UsnRNPs, transport of inner nuclear membrane proteins, nucleocytoplasmic transport of steroid hormone receptors, the role of the cytoskeleton in nucleocytoplasmic transport, and single substrate and transport receptor molecules at the nuclear pore
Recently, a group of researchers at Oxford University published a model for the structure of the nuclear pore
complex (NPC), the gatekeeper for the movement of molecules into and out of the nucleus (Alber et al., 2007a,b).
The overall size of the CNH is too large to navigate the smaller (~40 nm dia) nuclear pore
For example, those proteins specific to the nuclear envelope and components of the nuclear pore
complexes, which allow the transport of large molecules to and from the nucleus, are cobbled together from protein domains specific to eubacteria and archaea, with various eukaryotic innovations.