Nosema apis Zander, is a microsporidian pathogen of honey bees, Apis mellifera L., causing nosemosis in its host (Bailey & Ball 1991; Fries 1997).
DNA was extracted separately from individual worker honey bee thoraces (AHB/EHB testing) and abdomens (Nosema testing) using a salting-out procedure with in-house reagents (Sambrook & Russell 2001).
Molecular detection of Nosema was conducted using the associated abdominal DNA extractions, with PCR primers that were designed by finding conserved regions in aligned Nosema small subunit (SSU) sequences obtained from GenBank (Geneious v6.1.6, Biomatters, Auckland, New Zealand).
mellifera samples (245 AHB, 272 EHB), collected from Arkansas, Mississippi, Texas, Utah, Oklahoma, and New Mexico from 1991 to 2010, were subjected to PCR-RFLP analysis for molecular identification of Nosema apis and N.
Prevalence of Nosema from AHB was highest in Texas (12%) and lowest in New Mexico (3%), although there was no significant difference (P = 0.183).
We did not detect Nosema from the 1991 and 2004 Texas samples, but the small sample sizes (17 and 10, respectively) may be the reason for not detecting it.
Several statewide Nosema surveys have been conducted on managed honey bee colonies in the United States.
We found that Nosema was widespread throughout the southwestern and south central United States.
Nosema and tracheal mites in the North Central region --2008 survey.