northwestern blotting

northwestern blotting

A hybridisation technique for identifying protein/RNA interactions in which protein is run on a gel, blotted, and probed with a labelled RNA of interest. Interactions are detected as “hot” spots on a nitrocellulose filter (if the labelled probe is radioactive), or as dark spots (if the probe is biotinylated).
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Southern blotting was named after its developer, Edwin Southern; by analogy, similar conceptual approaches including Northern, Western, Southwestern, and Northwestern blotting were then named for compass directions).

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