The results of the present study reveal dose dependent significant decrease in the number of abnormal metaphases when norethynodrel (16 mg/ml) was treated with ascorbic acid (20, 40 and 80 mM) (Table 1).
The results in the present study clearly demonstrate that ascorbic acid is potent in reducing the genotoxic damage of norethynodrel. The metabolic activation of norethynodrel, may results in the generation of reactive species that are responsible for the genotoxic damage.
It is concluded that ascorbic acid it potent enough to reduced the genotoxic damage of norethynodrel in the presence of metabolic activation and may reduce the possible risk of carcinogenesis during the steroid therapy in patients.
Evaluation of genotoxic potential of norethynodrel in human lymphocytes in vitro.