was used as the negative control, and immune serum was obtained after seven days of the final immunization.
Negative controls included samples run without the primary antibody or with nonimmune serum
. Positive and negative controls supplied in the HercepTest kit were used for the evaluation of the HER2 stains.
cruzi in nonimmune serum
at early stages was demonstrated to be dependent on MASP2 and C2, which confirmed the involvement of the lectin pathway .
After nonspecific binding sites were blocked with 0.3% [H.sub.2][O.sub.2] in methanol for 30 minutes, the sections were incubated with normal nonimmune serum
for 1 hour.
Mouse polyclonal antibodies against JEV and WNV and nonimmune serum
samples were included in the assay.
A role for natural antibody in the pathogenesis of leprosy: antibody in nonimmune serum
mediates C3 fixation to the Mycobacterium leprae surface and hence phagocytosis by human mononuclear phagocytes.
Method controls to validate the specificity of the binding of immunocytochemical reagents with tissue included: 1) omission of the primary antiserum, 2) replacement of the primary antiserum with nonimmune serum
, 3) dilution profile of the primary antiserum using doubling dilutions on serial sections, 4) influence of the salt content (up to 0.5 m) of the buffer, and 5) complement-deprived antisera.
Equipment manufacturers, to protect their assays from these effects, include nonimmune serum
or immunoglobulin in their reagents, giving a very low incidence of TSH increase.
Sections were pretreated with nonimmune serum
(normal goat serum, 1: 20, for 15 minutes).
Controls consisted of replacement of the primary anti-serum with nonimmune serum
In contrast, injection of nonimmune serum
under the same conditions led to a low and similar reflectivity from all of the spots, which corresponded to nonspecific binding (background value).
Sections were processed using the procedure described herein or in reaction minus the primary antibody and with nonimmune serum
for the negative control.