Mouse polyclonal antibodies against JEV and WNV and nonimmune serum
samples were included in the assay.
Equipment manufacturers, to protect their assays from these effects, include nonimmune serum
or immunoglobulin in their reagents, giving a very low incidence of TSH increase.
In contrast, injection of nonimmune serum under the same conditions led to a low and similar reflectivity from all of the spots, which corresponded to nonspecific binding (background value).
3B, the curves featured similar profiles regardless of whether the screened antibodies were diluted in nonimmune serum or in buffer, confirming that the method could indeed be used for the analysis of a complex medium.
Another study investigated interferences in a two-site CK-MB assay and showed the broad reactivity of interfering globulins to nonimmune serum
from a diverse range of animals (Table 5) (89).
Interference by heterophile antibodies can usually be abolished or decreased by addition of either nonimmune serum
from the same animal species used to raise the antibody reagents or purified or polymerized homologous nonspecific immunoglobulin [3, 4, 66, 70, 73, 85,109-112].