nonadherent cell

nonadherent cell

[-ədhir′ənt]
a cell such as a lymphocyte that will not adhere to a smooth surface of laboratory equipment.
References in periodicals archive ?
The 26 chapters provide illustrated instructions and tips for avoiding common problems in reproducing clonogenic, colorimetric, fluorometric, and physiological assays in working with adherent/ nonadherent cell populations.
After incubation for 30 min at 37[degrees]C, the medium was discarded and the cells were washed twice with PBS to remove the nonadherent cells.
After a 1-h attachment period in the incubator, nonadherent cells, mainly MCs, were separated using Percoll density gradient centrifugation at 2500 g for 15 min at 4[degrees]C, and cells that remained at the Percoll interface were aspirated and resuspended in PBS.
After 72 hours, nonadherent cells were discarded, and the adherent ones were thoroughly washed twice with DMEM.
2], washed three times with HBSS to remove nonadherent cells, and equilibrated with DMEM that contained 10 per cent FBS before treatment.
After biofilm formation, the medium was aspirated and nonadherent cells were removed by thoroughly washing the biofilm.
2] at 37 [degrees]C for 5-7 clays; the nonadherent cells were then removed, whereas the plastic-adherent cells (the MSCs) were maintained under the same culture conditions.
Nonadherent cells were removed and adherent cells were cultured in 1 ml of fresh medium (5% FBS) containing 5 [micro]g/ml of E.
Nonadherent cells were removed 20 min after plating by changing the culture medium to DMEM/F12 containing 2% FBS.
for 20 min to remove nonadherent cells and erythrocytes and eluted adherent cells using a combination of high shear (flow rate 40 [micro]L/min), calcium-free PBS, and air embolism.