nonadherent cell

nonadherent cell

[-ədhir′ənt]
a cell such as a lymphocyte that will not adhere to a smooth surface of laboratory equipment.
References in periodicals archive ?
The 26 chapters provide illustrated instructions and tips for avoiding common problems in reproducing clonogenic, colorimetric, fluorometric, and physiological assays in working with adherent/ nonadherent cell populations.
Next, nonadherent cells were removed, fresh medium was added with 50 ng/mL of macrophage colony stimulating factor (MCSF) (Peprotech[R]), and the cells were cultured for 5 d.
Future applications will include single-cell protein analysis on CyTOF and culture of nonadherent cells, such as blood cells.
After washing away the nonadherent cells with PBS, the strips and the cells were incubated at 370 C to allow for biofilm formation.
After a 1-h attachment period in the incubator, nonadherent cells, mainly MCs, were separated using Percoll density gradient centrifugation at 2500 g for 15 min at 4[degrees]C, and cells that remained at the Percoll interface were aspirated and resuspended in PBS.
In primary cultures, the cells grew rapidly as such the media change for removal of nonadherent cells was carried out 48 hr of culture and 100% confluency was achieved on day 4 in liver and bone marrow MSCs and day 5 of the culture in A-MSCs (Figure 1).