nitroblue tetrazolium


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ni·tro·blue tet·ra·zo·li·um (NBT),

a pale yellow dye that is converted on reduction to colored formazans in the histochemical demonstration of dehydrogenases; used in hematology for staining of neutrophils to help indicate the presence of bacterial infections.
References in periodicals archive ?
(a) Succinate dehydrogenase (SDH) activity in liver protein extracts of ApoE KO mice was estimated with colorimetric method containing nitroblue tetrazolium reagents.
2 mL of clotted blood and 1 mL of fresh EDTA anticoagulated blood were collected for serum alpha-1 AT assay and nitroblue tetrazolium test respectively.
Laboratory evaluation for neutrophil's oxidative burst was performed and diagnosis of chronic granulomatous disease (CGD) was confirmed by both negative Nitroblue tetrazolium test (NBT) and lack of dihydrorhodamine (DHR) fluorescence following neutrophil stimulation against phorbol myristate acetate (PMA).
Chemical reagents including a JNK inhibitor (JNKI), brefeldin A (BFA), SP600125 (SP), BCIP, MTT, and nitroblue tetrazolium (NBT) were obtained from Sigma Chemical (St.
Fructosamine test was conducted using nitroblue tetrazolium method.
Immunologic studies including serum IgG IgA and IgM and phagocytic function tested with nitroblue tetrazolium were within normal limits.
2,6-Dichloro-indophenol (DFI), Folin-Ciocalteu Reagent, Formic acid, Acetonitrile, Anthrone, Cyanidin 3-rhamnoside, Quercetin 3-rhamnoside, Pelargonidin 3-rhamnoside, 2,2-Azinobis-3-ethylbenzthiazoline-6-sulphonic acid radical cation ([ABTS.sup.*+]), 6-Hydroxy-2,5,7,8tetramethylchroman-2-carboxylic acid (Trolox), Ethylenediaminetetracetic acid (EDTA), Nitroblue tetrazolium chloride (NBT) and Hydrogen peroxide ([H.sub.2][O.sub.2]) were purchased from Sigma Chemical Co.
Activity was determined by measuring its ability to inhibit the photochemical reduction of nitroblue tetrazolium chloride [22].
Pick, "Microassays for superoxide and hydrogen peroxide production and nitroblue tetrazolium reduction using an enzyme immunoassay microplate reader," Methods in Enzymology, vol.
Abbreviations BSA: Bovine serum albumin PAGE: Polyacrylamide gel electrophoresis NBT: Nitroblue tetrazolium PMS: Phenazine methosulphate PPP: Pentose phosphate pathway [NADP.sup.+]: [beta]-Nicotinamide adenine dinucleotide phosphate NADPH: Reduced [beta]-Nicotinamide adenine dinucleotide phosphate G6PD: Glucose-6-phosphate dehydrogenase.
Finally, the sections were kept in the dark at room temperature in the colour detection substrate solution nitroblue tetrazolium and 5-bromo-4-chloro-3'-Indolylphosphate (BCIP/NBT) as recommended by manufacturer' (Roche) in NTM until appearance of the colour.