nitroblue tetrazolium


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ni·tro·blue tet·ra·zo·li·um (NBT),

a pale yellow dye that is converted on reduction to colored formazans in the histochemical demonstration of dehydrogenases; used in hematology for staining of neutrophils to help indicate the presence of bacterial infections.

nitroblue tetrazolium

a yellow dye converted to a blue color on reduction.

nitroblue tetrazolium test
used to measure the phagocytic activity of polymorphonuclear leukocytes by the amount of color change in the dye.
References in periodicals archive ?
The principle of NBT test is detection of increased enzymatic activity of neutrophils, which is capable of reducing nitroblue tetrazolium to formazan, which forms a black precipitate.
1) activity was determined spectrophotometrically based on inhibition of the photochemical reduction of nitroblue tetrazolium chloride (NBT) (BEAUCHAMP; FRIDOVICH, 1971; GIANNOPOLITIS; RIES, 1977).
No obstante se sabe, que la aparicion de centros melanomacrofagos en la zona renal puede deberse a procesos inflamatorios y deposiciones de melanina, lipofuscina y hemosiderina los cuales han sido usados como bioindicadores de estres oxidativo, lo que puede explicarse debido al aumento en los niveles de explosion respiratoria expresados en la prueba con Nitroblue Tetrazolium (NBT) (7, 36).
The evaluation of a quantitative assay for estimating the bactericidal activity of bovine neutrophils by nitroblue tetrazolium reduction.
The method consists of a colorimetric determination of the ROSs produced by the leukocytes respiratory burst, which promotes reduction of nitroblue tetrazolium (NBT, Sigma, St.
Oncesinde buyume gelismesi normal olan, immun yetmezlik acisindan tekrarlayan enfeksiyon oykusu olmayan olguda aspergillus osteomiyeliti ve epidural apse gelismesi nedeniyle yapilan immunolojik tetkiklerinde immunglobulinleri normal sinirlarda, fagositleri stimule eden phorbol myristate acetate (PMA) ile stimule nitroblue tetrazolium (NBT) test yaniti cok dusuk (Normal>%90) bulundu, bu bulgularla olgumuz KGH tanisi aldi.
Initial evaluation for suspected CID should include a complete blood count, quantitative immunoglobulins, mitogen stimulation assay' tetanus titer, T- and B-cell flow cytometry' and nitroblue tetrazolium or chronic granulomatous disease flow assay, said Dr.
Initial evaluation for suspected CID should include a complete blood count, quantitative immunoglobulins, mitogen stimulation assay, tetanus titer, T- and B-cell flow cytometry, and nitroblue tetrazolium or chronic granulomatous disease flow assay, said Dr.
The patient's neutrophil phagocytic function, evaluated by the nitroblue tetrazolium test and complement assays (C3, C4 and CH50), was normal.
The assay of SOD activity was based on the principle of inhibitory effects of SOD on reduction of nitroblue tetrazolium dye.
For Superoxide radical scavenging activity, the superoxide radicals generated from dissolved oxygen by PMS=NADH coupling can be measured by their ability to reduce nitroblue tetrazolium.
Superoxide dismutase (SOD) activity was assayed at 520nm by the method of Kakkar et al, 1984 which was based on the inhibition of NADH- phenazine methosulphate nitroblue tetrazolium formation.