Neutrophil ingestion rate of nitroblue tetrazolium
in subjects with malaria-HIV co-morbidity.
The evaluation of a quantitative assay for estimating the bactericidal activity of bovine neutrophils by nitroblue tetrazolium
fructosamines) to reduce nitroblue tetrazolium
salts in alkaline solution (44).
The reduction of nitroblue tetrazolium
(NBT) to insoluble blue formazan was used to quantify intracellular superoxide anion ([O.
Superoxide dismutase activity was assayed by measuring the ability to inhibit the photochemical reduction of nitroblue tetrazolium
Alkaline phosphatase staining was developed with NBT solution (2 mg nitroblue tetrazolium
, 10 mg BCIP [5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt], 200 [micro]L dimethyl sulfoxide in 20 mL Tris HCl, pH 8.
18,19] Scherer-Singler et al described the enzymatic reduction of nitroblue tetrazolium
to an intensely blue, water-insoluble salt by nicotinamide adenine dinucleotide phosphate (reduced form, NADPH) diaphorase in a population of neurons.
A The fructosamine assay measures the ability of serum proteins with glucose attached to reduce nitroblue tetrazolium
1) was assayed by monitoring its ability to inhibit the photochemical reduction of nitroblue tetrazolium
1998, 2000a), but also non-specific immune response (lysozyme activity and nitroblue tetrazolium
reduction of macrophage) (Park et al.
8% agarose gel in Tris-acetate buffer, transferred onto a positively charged nylon membrane (Roche Diagnostics), fixed to the membrane by baking for 30 min at 120[degrees]C, hybridized with a digoxigenin-labeled probe complementary to 16S and 23S rRNA genes of Escherichia coli and detected with alkaline phosphatase-labeled antidigoxigenin and nitroblue tetrazolium
and 5-bromo-4-chloro-3-indolyl-phosphate (Roche Diagnostics).
After rinsing twice in 1x phosphate-buffered saline for 5 minutes, we prepared a substrate buffer by diluting nitroblue tetrazolium