ninhydrin reaction


Also found in: Encyclopedia.

nin·hy·drin re·ac·tion

a test for proteins, peptones, peptides, and amino acids possessing free carboxyl and α-amino groups that is based upon the reaction with triketohydrindene hydrate; a blue color reaction is used to quantitate free amino acids (for example, after hydrolysis and separation of the amino acids of a protein).

nin·hy·drin re·ac·tion

(nin-hī'drin rē-ak'shŭn)
A procedure for proteins, peptones, peptides, and amino acids possessing free carboxyl and α-amino groups that is based on the reaction with triketohydrinene hydrate; a blue color reaction is used to quantitate free amino acids.
Mentioned in ?
References in periodicals archive ?
The supernatant of each sample was used to measure the protein and AAs concentrations by performing the Lowry method and ninhydrin reaction, respectively [37].
Amino acid in sweat can be detected using ninhydrin reaction. Ninhydrin (2,2-dihydroxyindane-1,3-dione) is a chemical used to detect ammonia or primary and secondary amines.
Proline content was estimated using ninhydrin reaction (Bates et al.
As a result, it was confirmed that much protein was included in carbonate warm water by a ninhydrin reaction.
The A spot was positive for both Millon's and the Ninhydrin reaction. The C spot was positive for both Millon's and the phosphomolibdic acid reaction.
The concentration of free amino groups in TCA soluble protein was determined with the ninhydrin reaction by spectrophotometer (Sarin et al., 1981).
Initially, tHcy was measured with amino acid analyzers, using the ninhydrin reaction as the method of detection.
Experiments with and without leaf tissue confirmed that the interference was nonenzymatic and that [MATHEMATICAL EXPRESSION NOT REPRODUCIBLE IN ASCII] interfered with the ninhydrin reaction. The interference was highly dependent on the ratio of the nitrate/proline concentration.