nickase

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nickase

A popular term for a restriction endonuclease that introduces a break in one strand of double-stranded DNA.

References in periodicals archive ?

Single nucleotide mutation in two domains (RuvC, HNH) of Cas9 transforms it into Cas9 nickase [5, 9].

CRISPR CAS9 SYSTEM: A NOVEL GENOME EDITING TOOL

Cas9 specificity was increase 100-1500 fold by the use of a pair of Cas9 nickase variants, which were directed to target on opposite strands up to 100 bp apart.

RNA-guided genome editing tool CRISPR-Cas9: its applications and achievements in model and crop plants

When combined with pairs of guide RNAs targeting nearby sites on opposite strands, the nickase approach greatly improved target site specificity.

CRISPR-Cas9 System: Opportunities and Concerns

However, the mutant nickase creates a single-strand DNA break at the based on gRNA-defined target sequence [12].

Myostatin gene knockout mediated by Cas9-D10A nickase in chicken DF1 cells without off-target effect

The molecular biology grade ethanol has no detectable Dnase, Rnase or Nickase activity.

Product Showcase

Use-tested for nucleic acid precipitation, it has no detectable DNase, RNase, or Nickase activity.

Pittcon 2000 New Products

Use-tested for nucleic acid precipitation, Molecular Biology Grade Ethanol has no detectable Dnase, Rnase or Nickase activity.

Editor's Choice

Analysis of off-target effects of CRISPR/ Cas-derived RNA-guided endonucleases and nickases.

Human lactoferrin efficiently targeted into caprine beta-lactoglobulin locus with transcription activator-like effector nucleases

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.