nickase


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nickase

A popular term for a restriction endonuclease that introduces a break in one strand of double-stranded DNA.
References in periodicals archive ?
Single nucleotide mutation in two domains (RuvC, HNH) of Cas9 transforms it into Cas9 nickase [5, 9].
Cas9 specificity was increase 100-1500 fold by the use of a pair of Cas9 nickase variants, which were directed to target on opposite strands up to 100 bp apart.
When combined with pairs of guide RNAs targeting nearby sites on opposite strands, the nickase approach greatly improved target site specificity.
However, the mutant nickase creates a single-strand DNA break at the based on gRNA-defined target sequence [12].
The molecular biology grade ethanol has no detectable Dnase, Rnase or Nickase activity.
Use-tested for nucleic acid precipitation, it has no detectable DNase, RNase, or Nickase activity.
Use-tested for nucleic acid precipitation, Molecular Biology Grade Ethanol has no detectable Dnase, Rnase or Nickase activity.
Analysis of off-target effects of CRISPR/ Cas-derived RNA-guided endonucleases and nickases.
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.