nick translation


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Related to nick translation: Klenow fragment

nick trans·la·tion

a technique in which a bacterial DNA polymerase is used to degrade a single strand of DNA that has been nicked and then to resynthesize that strand, often with labeled nucleoside triphosphates.

nick translation

a process of repairing a NICK in a DNA MOLECULE using the enzyme DNA POLYMERASE. This method is often used in vitro to introduce labelled NUCLEOTIDES into DNA in order to label a DNA PROBE.
References in periodicals archive ?
Differentiation between cellular apoptosis and necrosis by the combined use of in situ tailing and nick translation techniques.
Amplified inserts were biotin-labelled by nick translation (Kelly et al., 1970; Rigby et al., 1977), using reagents from Bethesda Research Laboratories.
Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.